Cloning and Characterization of a GmSAGT1 Gene Whose Transcriptional Expression Is Associated with the Level of Resistance to Soybean Downy Mildew

文献类型： 外文期刊

第一作者： Sun, Wenli

作者： Sun, Wenli;Yin, Chao;Li, Mei;Zhang, Huaguang;Shi, Shuangfeng;Jia, Shirong;Wang, Zhixing;Liu, Yuhui;Sun, Wenli;Wu, Yuanhua;Wu, Qiao

作者机构：

关键词： Soybean;Downy mildew;Salicylic acid;Serine/alanine glyoxylate aminotransferase;Hydrogen peroxide (H2O2)

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We have cloned a GmSAGT1 gene coding for a bi-functional enzyme of serine/alanine glyoxylate aminotransferase from a soybean variety Zaofeng5 resistant to soybean downy mildew (SDM). The GmSAGT1 expressed in Escherichia coli exhibited serine glyoxylate aminotransferase (SGT) and alanine glyoxylate aminotransferase (AGT) activity that produced 20-fold more glycine than that of the control. The amount of hydrogen peroxide (H2O2) dramatically increased in E. coli expressing GmSAGT1, and bacterial growth was severely inhibited. Real-time polymerase chain reaction (RT-PCR) showed that GmSAGT1 could be detected in Zaofeng5 prior to salicylic acid (SA) treatment and increased by fivefold after SA induction. In contrast, it was not detectable in susceptible variety Heinong10 prior to and only slightly increased after SA induction. Fifteen soybean varieties belonging to highly resistant (HR), medium resistant (MR), resistant (R), susceptible (S), and highly susceptible (HS) category were used for real-time quantitative RT-PCR (qRT-PCR) analysis, which showed the average expression level of GmSAGT1 in HR group was 13 times of that in HS group. Northern blot analysis indicated that the expression level of GmSAGT1 in HS, S, MR, and R group was 5, 22, 39, and 65 % of that in HR, respectively. Transgenic tobacco expressing GmSAGT1 increased resistance to a fungal pathogen Rhizoctonia solani. Our results have demonstrated that higher expression of GmSAGT1 gene is closely correlated to increased SDM resistance.

分类号： Q94`Q7

  • 相关文献

[1]Distribution of Baseline Sensitivities to Natural Product Physcion Among Isolates of Sphaerotheca fuliginea and Pseudoperonospora cubensis. Yang, X. J.,Ni, H.,Yang, X. J.,Yang, L. J.,Zeng, F. S.,Xiang, L. B.,Wang, S. N.,Yu, D. Z..

[2]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[3]The Arabidopsis J-protein AtDjB1 facilitates thermotolerance by protecting cells against heat-induced oxidative damage. Zhou, Wei,Zhou, Ting,Li, Mi-Xin,Zhao, Chun-Lan,Jia, Ning,Wang, Xing-Xing,Sun, Yong-Zhen,Xu, Meng,Li, Bing,Zhou, Wei,Li, Guo-Liang,Zhou, Ren-Gang,Zhou, Wei. 2012

[4]Novel roles of hydrogen peroxide (H2O2) in regulating pectin synthesis and demethylesterification in the cell wall of rice (Oryza sativa) root tips. Xiong, Jie,Yang, Yongjie,Fu, Guanfu,Tao, Longxing,Xiong, Jie.

[5]Guard cell hydrogen peroxide and nitric oxide mediate elevated CO2-induced stomatal movement in tomato. Shi, Kai,Li, Xin,Zhang, Huan,Zhang, Guanqun,Liu, Yaru,Zhou, Yanhong,Xia, Xiaojian,Chen, Zhixiang,Yu, Jingquan,Shi, Kai,Yu, Jingquan,Li, Xin,Chen, Zhixiang.

[6]Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape. Fan, J. J.,Xu, X.,Ruan, Y. Y.,Zhu, Y. S.,Cui, Z. H.,Zhang, L. J.,Wang, P.,Liu, K.. 2015

[7]Breeding disease-resistant Hami-melon. Wu, MZ,Yi, HP,Zhai, WQ,Feng, JX. 2004

[8]Sensitivity of Pseudoperonospora cubensis to dimethomorph, metalaxyl and fosetyl-aluminium in Shanxi of China. Zhao, Xiaojun,Ren, Lu,Han, Jvcai,Zhao, Xiaojun,Yin, Hui,Zhou, Jianbo,Luo, Yong. 2013

[9]Expression analysis of major genes involved in signaling pathways during infection of Chinese cabbage with Hyaloperonospora brassicae. Gao, Tingting,Yu, Shuancang,Zhang, Fenglan,Yu, Yangjun,Zhang, Deshuang,Zhao, Xiuyun,Wang, Weihong,Gao, Tingting,Chen, Xuehao. 2014

[10]Molecular cloning of a CC-NBS-LRR gene from Vitis quinquangularis and its expression pattern in response to downy mildew pathogen infection. Ding, Feng,Lu, Jiang,Peng, Hongxiang,Huang, Yu. 2018

[11]HISTOCHEMICAL RESPONSES OF LEAVES OF IN-VITRO PLANTLETS OF VITIS SPP TO INFECTION WITH PLASMOPARA-VITICOLA. DAI, GH,ANDARY, C,MONDOLOTCOSSON, L,BOUBALS, D. 1995

[12]Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.). Xu Liang,Jiang Qiu-wei,Wang Yan,Gong Yi-qin,Wang Xian-li,Limera Cecilia,Liu Li-wang,Wu Jian. 2014

[13]Isolation of resistance gene analogs from grapevine resistant to downy mildew. Wang, Ping,Wang, Dongxu,Liang, Chunhao,Fan, Jinjuan,Wang, Ping,Liu, Changyuan,Liang, Chunhao,Zhao, Kuihua. 2013

[14]Proteomic Analysis of the Resistant Responses of Two Vitis amurensis Cultivars to Plasmopara viticola Infections. Xu, Pei-lei,Liu, Ying-xue,Qin, Hong-yan,Ai, Jun,Fan, Shu-tian,Yang, Yi-ming,Zhao, Ying,Li, Xiao-yan,Li, Xiao-hong.

[15]Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance. Jiang Ming,He Cai-ming,Miao Li-xiang,Zhang Yu-chao. 2012

[16]Characterization of the secretome of Plasmopara viticola by de novo transcriptome analysis. Li, Xinlong,Xiang, Jiang,Zhang, Yali,Lu, Jiang,Yin, Ling,Dry, Ian B.,Qu, Junjie.

[17]Construction of a sequence-based bin map and mapping of QTLs for downy mildew resistance at four developmental stages in Chinese cabbage (Brassica rapa L. ssp pekinensis). Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun,Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun,Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun.

[18]Comparative transcriptome analysis reveals defense-related genes and pathways against downy mildew in Vitis amurensis grapevine. Wu, Jiao,Yin, Ling,Zhang, Yali,Lu, Jiang,Qu, Junjie.

[19]Overexpression of an Oil Radish Superoxide Dismutase Gene in Broccoli Confers Resistance to Downy Mildew. Jiang, Ming,He, Caiming,Miao, Li-xiang.

[20]Trichokonins from Trichoderma pseudokoningii SMF2 induce resistance against Gram-negative Pectobacterium carotovorum subsp carotovorum in Chinese cabbage. Li, Hai-Yun,Luo, Yan,Shi, Wei-Ling,Gong, Zhi-Ting,Shi, Mei,Chen, Xiu-Lan,Zhang, Yu-Zhong,Song, Xiao-Yan,Li, Hai-Yun,Zhang, Xiu-Sheng,Chen, Lei-Lei. 2014

作者其他论文 更多>>

微信小程序