Threonine influences the absorption capacity and brush-border enzyme gene expression in the intestine of juvenile blunt snout bream (Megalobrama amblycephala)

文献类型： 外文期刊

第一作者： Habte-Tsion, Habte-Michael

作者： Habte-Tsion, Habte-Michael;Ren, Mingchun;Liu, Bo;Ge, Xianping;Xie, Jun;Zhou, Qunlan;Ren, Mingchun;Liu, Bo;Ge, Xianping;Xie, Jun;Chen, Ruli;Zhou, Qunlan;Pan, Liangkun;Habte-Tsion, Habte-Michael

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关键词： Megalobrama amblycephala;Threonine;Intestine;Enzyme activity;Brush-border enzyme gene expression

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

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收录情况： SCI

摘要： A 9-week experiment was conducted to investigate the effects of dietary threonine levels on the enzyme activities, plasma ammonia content and intestinal brush-border enzyme gene expressions in juvenile blunt snout bream. Five isonitrogenous and isoenergetic semi-purified diets (34% crude protein) were formulated to contain graded levels of dietary threonine (0.6 (un-supplemented control), 1.1, 1.6, 2.1 and 2.6% in dry matter). The diets were supplemented with 0, 0.5, 1.0, 1.5 or 2.0% L-crystalline threonine. L-Amino acid mixtures were prepared taking into account the amount of amino acids contributed by fish meal, casein and gelatin. Juvenile fish (initial weight, 3.01 +/- 0.01 g) were assigned into triplicate groups and fed with their respective diet to near satiation four times per day. The results indicated that the activities of alkaline phosphatase (AKP), sodium/potassium-ATPase transporter (Na+/K+-ATPase) and gamma-glutamyl transpeptidase (gamma-GT) in the three intestinal segments rose as dietary threonine levels increased and reached a pick value at 1.58% dietary threonine and thereafter decreased. The highest glutamate-oxaloacetate transaminase (GOT) activity in hepatopancreas was found in the group fed with high (2.58%) threonine diet (P < 0.05). Glutamate-pyruvate transaminase (GPT) activity in hepatopancreas and plasma ammonia content (PAC) exhibited significant (P < 0.05) "V-shaped" trends with increasing dietary threonine levels and the lowest values were found at 1.58% threonine, while plasma alkaline phosphatase (ALP) didn't significantly respond to threonine supplementation. Dietary threonine regulated the gene expressions of brush-border enzymes (AKP, Na+/K+-ATPase and gamma-GT) in the intestinal segments, which may explain further that threonine enhanced the absorptive function of fish via regulating brush-border enzyme synthesis and secretion.

分类号： S9

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