Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis
文献类型: 外文期刊
第一作者: Zhou, Meiliang
作者: Zhou, Meiliang;Sun, Zhanmin;Wang, Chenglong;Tang, Yixiong;Wu, Yanmin;Wang, Chenglong;Shao, Jirong;Zhang, Xinquan;Zhu, Xuemei
作者机构:
关键词: Arabidopsis thaliana;bimolecular fluorescence complementation;GY;FDFLGL motif;MYB transcription factor;phenylpropanoid pathway;point mutation;transcriptional repressor
期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Sub-group4 R2R3-type MYB transcription factors, including MYB3, MYB4, MYB7 and MYB32, act as repressors in phenylpropanoid metabolism. These proteins contain the conserved MYB domain and the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) repression domain. Additionally, MYB4, MYB7 and MYB32 possess a putative zinc-finger domain and a conserved GY/FDFLGL motif in their C-termini. The protein sensitive to ABA and drought 2' (SAD2) recognizes the nuclear pore complex, which then transports the SAD2-MYB4 complex into the nucleus. Here, we show that the conserved GY/FDFLGL motif contributes to the interaction between MYB factors and SAD2. The AspAsn mutation in the GY/FDFLGL motif abolishes the interaction between MYB transcription factors and SAD2, and therefore they cannot be transported into the nucleus and cannot repress their target genes. We found that MYB4(D261N) loses the capacity to repress expression of the cinnamate 4-hydroxylase (C4H) gene and biosynthesis of sinapoyl malate. Our results indicate conservation among MYB transcription factors in terms of their interaction with SAD2. Therefore, the AspAsn mutation may be used to engineer transcription factors.
分类号: Q94
- 相关文献
作者其他论文 更多>>
-
Plant- and microbial-mediated soil organic carbon accumulation in Spartina alterniflora salt marshes
作者:Liao, Qihang;Yuan, Feng;Fan, Qinya;Chen, Hongyu;Wang, Yameng;Zhang, Chuchu;Wang, Chenglong;Zou, Xinqing;Liao, Qihang;Yuan, Feng;Fan, Qinya;Chen, Hongyu;Wang, Yameng;Zhang, Chuchu;Wang, Chenglong;Zou, Xinqing;Fan, Qinya;Chen, Hongyu;Zou, Xinqing;Lu, Chao;Qiu, Penghua;Zou, Xinqing
关键词:Coastal wetland; Chemical stability; Plant; and microbial-derived carbon; Spartina alterniflora invasion; Soil organic carbon; Tide
-
Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat
作者:He, Yuqi;Zhang, Kaixuan;Shi, Yaliang;Lin, Hao;Huang, Xu;Lu, Xiang;Wang, Zhirong;Li, Wei;Tang, Yu;Liu, Xu;Zhou, Meiliang;Feng, Xibo;Shi, Taoxiong;Chen, Qingfu;Wang, Junzhen;Chapman, Mark A.;Germ, Mateja;Luthar, Zlata;Kreft, Ivan;Janovska, Dagmar;Meglic, Vladimir;Woo, Sun-Hee;Quinet, Muriel;Fernie, Alisdair R.
关键词:Domestication; Migration; Artificial selection; Buckwheat; Genomics
-
Evolutionary analysis of MADS-box genes in buckwheat species and functional study of FdMADS28 in flavonoid metabolism
作者:Liu, Yang;Guan, Chaonan;Zhou, Meiliang;Liu, Yang;Guan, Chaonan;Shi, Yaliang;Long, Ou;Lin, Hao;Zhang, Kaixuan;Zhou, Meiliang;Chen, Yuanyuan
关键词:Buckwheat; MADS-Box; Flavonoid; Stress resistance
-
Comparison of Morphometric Parameters, Nutritional Composition, and Textural Properties of Seven Crustaceans Species
作者:Yang, Zhigang;Jiang, Qingqing;Chen, Aqin;Jiang, Qingqing;Zhang, Wuxiao;Xia, Silei;Tian, Hongyan;Liu, Fei;Yang, Wenping;Yu, Yebing;Wu, Yanmin;Wang, Aimin;Zhu, Yongzhi;Xu, Zhiqiang;Gu, Zemao
关键词:muscle quality; amino acid composition; fatty acid composition; textural properties; crustaceans
-
Comparative Genomics of Lotus japonicus Reveals Insights into Proanthocyanidin Accumulation and Abiotic Stress Response
作者:Sun, Zhanmin;Liu, Ziyang;Zhi, Manqing;Tang, Yixiong;Wu, Yanmin;Ran, Qifan;Xue, Wenbo
关键词:Lotus japonicus; proanthocyanidins; RNA-seq; abiotic stress; comparative genomics
-
Selection and Validation of Reference Genes in Sudan Grass (Sorghum sudanense (Piper) Stapf) under Various Abiotic Stresses by qRT-PCR
作者:Wang, Fangyan;Li, Peng;Nie, Gang;Zhang, Xinquan;Liu, Qiuxu;Zhu, Yongqun
关键词:reference gene; Sudan grass; qRT-PCR; abiotic stress
-
Effect of Biogas Slurry on the Soil Properties and Microbial Composition in an Annual Ryegrass-Silage Maize Rotation System over a Five-Year Period
作者:Feng, Guangyan;Hao, Feixiang;Nie, Gang;Huang, Linkai;Wang, Xia;Yuan, Suhong;Zhang, Xinquan;Hao, Feixiang;Xu, Wenzhi;He, Wei;Ran, Qifan
关键词:biogas slurry; bacterial community; fungal community; soil chemistry; annual ryegrass-silage maize; soil