Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis

文献类型： 外文期刊

第一作者： Zhou, Meiliang

作者： Zhou, Meiliang;Sun, Zhanmin;Wang, Chenglong;Tang, Yixiong;Wu, Yanmin;Wang, Chenglong;Shao, Jirong;Zhang, Xinquan;Zhu, Xuemei

作者机构：

关键词： Arabidopsis thaliana;bimolecular fluorescence complementation;GY;FDFLGL motif;MYB transcription factor;phenylpropanoid pathway;point mutation;transcriptional repressor

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

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收录情况： SCI

摘要： Sub-group4 R2R3-type MYB transcription factors, including MYB3, MYB4, MYB7 and MYB32, act as repressors in phenylpropanoid metabolism. These proteins contain the conserved MYB domain and the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) repression domain. Additionally, MYB4, MYB7 and MYB32 possess a putative zinc-finger domain and a conserved GY/FDFLGL motif in their C-termini. The protein sensitive to ABA and drought 2' (SAD2) recognizes the nuclear pore complex, which then transports the SAD2-MYB4 complex into the nucleus. Here, we show that the conserved GY/FDFLGL motif contributes to the interaction between MYB factors and SAD2. The AspAsn mutation in the GY/FDFLGL motif abolishes the interaction between MYB transcription factors and SAD2, and therefore they cannot be transported into the nucleus and cannot repress their target genes. We found that MYB4(D261N) loses the capacity to repress expression of the cinnamate 4-hydroxylase (C4H) gene and biosynthesis of sinapoyl malate. Our results indicate conservation among MYB transcription factors in terms of their interaction with SAD2. Therefore, the AspAsn mutation may be used to engineer transcription factors.

分类号： Q94

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