Involvement of CD16 in antibody-dependent enhancement of porcine reproductive and respiratory syndrome virus infection

文献类型： 外文期刊

第一作者： Gu, Weihong

作者： Gu, Weihong;Guo, Longjun;Yu, Haidong;Niu, Junwei;Huang, Mingming;Luo, Xiaolei;Li, Ren;Tian, Zhijun;Feng, Li;Wang, Yue

作者机构：

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The immunological effect of porcine reproductive and respiratory syndrome disease virus (PRRSV) vaccines is thought to be influenced by a variety of host factors, in which antibody-dependent enhancement (ADE) of infection is one crucial factor. Here, we assessed the mechanism of ADE of PRRSV infection. First, we found that subneutralizing serum could induce ADE of PRRSV infection in porcine alveolar macrophages (PAMs). Quantitative PCR, Western blotting and flow cytometry revealed that CD16 is the most abundant Fc gamma receptor (Fc gamma R) expressed on the surface of PAMs; thus, the role of CD16 in ADE of PRRSV infection was examined in PAMs. By using functional blocking antibodies, we demonstrated that CD16 is involved in enhanced virus production in PRRSV antibody immune complex-infected PAMs. Because PAMs co-express different Fc gamma R isoforms, we evaluated the effects of CD16 in Fc gamma R-non-bearing cells by transfection. Using these engineered cells, we found that CD16 could specifically bind to the PRRSV antibody immune complex and subsequently mediate internalization of the virus, resulting in the generation of progeny virus. We also showed that efficient expression of CD16 required association of the FcR gamma-chain. Together, our findings provide significant new insights into PRRSV infection, which can be enhanced by CD16-mediated PRRSV antibody immune complexes. This CD16-mediated ADE may induce a shift in PRRSV tropism towards CD16-expressing cells, distributing virus to more organs during virus infection.

分类号： R37

  • 相关文献
作者其他论文 更多>>

微信小程序