Mutation of the rice ASL2 gene encoding plastid ribosomal protein L21 causes chloroplast developmental defects and seedling death

文献类型: 外文期刊

第一作者: Lin, D.

作者: Lin, D.;Jiang, Q.;Zheng, K.;Chen, S.;Zhou, H.;Gong, X.;Dong, Y.;Chen, S.;Zhou, H.;Teng, S.;Xu, J.

作者机构:

关键词: Chloroplast development;chloroplast ribosomal protein;gene mutation;map-based cloning;rice (Oryza sativa L;)

期刊名称:PLANT BIOLOGY ( 影响因子:3.081; 五年影响因子:2.972 )

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收录情况: SCI

摘要: The plastid ribosome proteins (PRPs) play important roles in plastid protein biosynthesis, chloroplast differentiation and early chloroplast development. However, the specialised functions of individual protein components of the chloroplast ribosome in rice (Oryza sativa) remain unresolved. In this paper, we identified a novel rice PRP mutant named asl2 (Albino seedling lethality 2) exhibiting an albino, seedling death phenotype. In asl2 mutants, the alteration of leaf colour was associated with chlorophyll (Chl) content and abnormal chloroplast development. Through map-based cloning and complementation, the mutated ASL2 gene was isolated and found to encode the chloroplast 50S ribosome protein L21 (RPL21c), a component of the chloroplast ribosome large subunit, which was localised in chloroplasts. ASL2 was expressed at a higher level in the plumule and leaves, implying its tissue-specific expression. Additionally, the expression of ASL2 was regulated by light. The transcript levels of the majority of genes for Chl biosynthesis, photosynthesis and chloroplast development were strongly affected in asl2 mutants. Collectively, the absence of functional ASL2 caused chloroplast developmental defects and seedling death. This report establishes the important role of RPL21c in chloroplast development in rice.

分类号: Q94

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