Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Zhao-Qun Li

作者： Zhao-Qun Li;Shuai Zhang;Jun-Yu Luo;Jing Zhu;Jin-Jie Cui;Shuang-Lin Dong

作者机构：

关键词： Helicoverpa armigera;Chemosensory proteins;Fluorescence binding assay;In Situ hybridization

期刊名称：JOURNAL OF CHEMICAL ECOLOGY （ 影响因子：2.626； 五年影响因子：2.984 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals to receptors on sensory neurons. We identified and cloned 24 CSP genes to better understand the physiological function of CSPs in Helicoverpa armigera. Quantitative real-time polymerase chain reaction assays indicate that CSP genes are ubiquitously expressed in adult H. armigera tissues. Broad expression patterns in adult tissues suggest that CSPs are involved in a diverse range of cellular processes, including chemosensation as well as other functions not related to chemosensation. The H. armigera CSPs that were highly transcribed in sensory organs or pheromone glands (HarmCSPs 6, 9, 18, 19), were recombinantly expressed in bacteria to explore their function. Fluorescent competitive binding assays were used to measure the binding affinities of these CSPs against 85 plant volatiles and 4 pheromone components. HarmCSP6 displays high binding affinity for pheromone components, whereas the other three proteins do not show affinities for any of the compounds tested. HarmCSP6 is expressed in numerous cells located in or close to long sensilla trichodea on the antennae of both males and females. These results suggest that HarmCSP6 may be involved in transporting female sex pheromones in H. armigera.

分类号： Q14

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