A long-lasting protective immunity against chronic toxoplasmosis in mice induced by recombinant rhoptry proteins encapsulated in poly (lactide-co-glycolide) microparticles

文献类型： 外文期刊

第一作者： Xu, Ying

作者： Xu, Ying;Zhang, Nian-Zhang;Wang, Meng;Dong, Hu;Feng, Sheng-Yong;Guo, Hui-Chen;Zhu, Xing-Quan;Xu, Ying;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis;Rhoptry protein 38;Rhoptry protein 18;Poly (lactide-co-glycolide) microparticles;Protective immunity

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Toxoplasma gondii infection in humans and animals is a worldwide zoonosis. Prevention and control of toxoplasmosis based on vaccination is one of the promising strategies. In the present study, recombinant T. gondii rhoptry proteins 38 and 18 (TgROP38 and TgROP18) were encapsulated into poly (lactide-co-glycolide) (PLG) (1:1), respectively, to obtain the stable water-in-oil-in-water double emulsion. Female Kunming mice were then immunized with the protein vaccines twice at a 2-week interval. Eight weeks after the second immunization, 10 mice from each group were challenged with T. gondii PRU strain (genotype II). The entrapment rates of PLG-rROP38 and PLG-rROP18 ranged from 65.5 to 77.7 % and 58.1 to 72.3 %, respectively. Immunization of mice with rROP38 and rROP18 proteins encapsulated into PLG microparticles elicited strongly humoral and cell-mediated responses against T. gondii, associated with relatively high levels of total IgG, IgG2a isotype, and IFN-gamma, as well as the mixed Th1/Th2 immunity responses. Immunization with various protein vaccines induced significant reduction of the brain cysts after chronic infection with the T. gondii PRU strain, and the most effective protection was achieved in the PLG-rROP38-rROP18-immunized mice, with a cyst reduction of 81.3 %. The findings of the present study indicated that recombinant rhoptry antigens encapsulated in PLG could maintain the protein immunogenicity in an extended period and elicit effective protection against chronic T. gondii infection, which has implications for the development of long-lasting vaccines against chronic toxoplasmosis in animals.

分类号： R53

  • 相关文献

[1]Vaccination with Toxoplasma gondii calcium-dependent protein kinase 6 and rhoptry protein 18 encapsulated in poly(lactide-co-glycolide) microspheres induces long-term protective immunity in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Gao, Qi,Huang, Si-Yang,Zhu, Xing-Quan. 2016

[2]Protective efficacy of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhou, Dong-Hui,Zhu, Xing-Quan,Chen, Jia,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild. 2014

[3]Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Li, Zhong-Yuan,Yuan, Zi-Guo,Zhou, Dong-Hui,Zhang, Nian-Zhang,Zhu, Xing-Quan,Chen, Jia,Li, Zhong-Yuan,Yuan, Zi-Guo,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[4]Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice. Li, Zhong-Yuan,Chen, Jia,Zhou, Dong-Hui,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild.

[5]DNA vaccination with genes encoding Toxoplasma gondii antigens ROP5 and GRA15 induces protective immunity against toxoplasmosis in Kunming mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Zhou, Dong-Hui,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild,Zhu, Xing-Quan. 2015

[6]DNA immunization with eukaryotic initiation factor-2 alpha of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Zhou, Dong-Hui,Li, Zhong-Yuan,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[7]Protective immunity against Toxoplasma gondii induced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3. Zhang, Nian-Zhang,Huang, Si-Yang,Zhou, Dong-Hui,Chen, Jia,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan,Tian, Wei-Peng,Lu, Jing,Zhu, Xing-Quan. 2013

[8]Evaluation of protective effect of pVAX-TgMIC13 plasmid against acute and chronic Toxoplasma gondii infection in a murine model. Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Zhou, Yang,Zhu, Xing-Quan,Yuan, Zi-Guo,Ren, Di,Zhang, Xiu-Xiang,Li, Xiu-Zhen,Petersen, Eskild,Petersen, Eskild,Yang, Gui-Lian,Zhu, Xing-Quan. 2013

[9]Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Chen, Kai,Wang, Jin-Lei,Huang, Si-Yang,Yang, Wen-Bin,Zhu, Wei-Ning,Zhu, Xing-Quan,Huang, Si-Yang,Zhu, Xing-Quan,Yang, Wen-Bin,Zhu, Wei-Ning. 2017

[10]Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Chen, Jia,Li, Zhong-Yuan,Liu, Wen-Ge,Zhu, Xing-Quan,Chen, Jia,Petersen, Eskild,Zhu, Xing-Quan.

[11]Evaluation of protective immunity induced by DNA vaccination with genes encoding Toxoplasma gondii GRA17 and GRA23 against acute toxoplasmosis in mice. Zhu, Wei-Ning,Wang, Jin-Lei,Chen, Kai,Yue, Dong-Mei,Zhang, Xiao-Xuan,Huang, Si-Yang,Zhu, Xing-Quan,Zhu, Wei-Ning. 2017

[12]Evaluation of immuno-efficacy of a novel DNA vaccine encoding Toxoplasma gondii rhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model. Xu, Ying,Zhang, Nian-Zhang,Tan, Qi-Dong,Chen, Jia,Lu, Jing,Zhu, Xing-Quan,Xu, Ying,Tan, Qi-Dong,Xu, Qian-Ming,Zhu, Xing-Quan. 2014

[13]Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice. Yang, Wen-Bin,Zhao, Guang-Hui,Yang, Wen-Bin,Zhou, Dong-Hui,Zou, Yang,Chen, Kai,Wang, Jin-Lei,Zhu, Xing-Quan,Liu, Qing,Zhu, Xing-Quan. 2017

[14]Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Petersen, Eskild,Huang, Si-Yang,Zhu, Xing-Quan. 2015

[15]Toxoplasma gondii: Protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis. Chen, Jia,Zhou, Dong-Hui,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Li, Zhong-Yuan,Zhu, Xing-Quan. 2014

[16]Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations. Ning, H. R.,Zhu, X. Q.,Ning, H. R.,Wang, J. L.,Qin, S. Y.,Huang, S. Y.,Lou, Z. L.,Hu, L. Y.,Zhu, X. Q.,Qin, S. Y.,Lou, Z. L.,Huang, S. Y.,Zhu, X. Q.. 2015

[17]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[18]Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations. Ren, Di,Zhou, Yang,Lin, Ze-Ping,Lin, Rui-Qing,Wu, Song-Ming,Lin, Shao-Qi,Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Xu, Min-Jun,Zhou, Yang,Wu, Song-Ming,Yuan, Zi-Guo,Yang, Jian-Fa,Zou, Feng-Cai. 2012

[19]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[20]Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. Zhao, Yu,Sun, Xiaolin,Zhao, Yu,Zhou, Donghui,Chen, Jia. 2017

作者其他论文 更多>>

微信小程序