Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

文献类型: 外文期刊

第一作者: Xia, Jin-lan

作者: Xia, Jin-lan;Nie, Zhen-yuan;Shan, Yang

作者机构:

关键词: Multi-enzyme complexes;Solid-state fermentation;Submerged fermentation;Ultrasound;Zymography

期刊名称:BIOPROCESS AND BIOSYSTEMS ENGINEERING ( 影响因子:3.21; 五年影响因子:2.97 )

ISSN:

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收录情况: SCI

摘要: Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

分类号: Q7

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