Cloning and expression of mink (Neovison vison) interferon-gamma gene and development of an antiviral assay
文献类型: 外文期刊
第一作者: Zhang, Hailing
作者: Zhang, Hailing;Zhao, Jianjun;Bai, Xue;Zhang, Lei;Hu, Bo;Liu, Hao;Xu, Shujuan;Yan, Xijun;Zhang, Hailing;Yan, Xijun;Zhang, Dongliang;Fan, Sining
作者机构:
关键词: Mink;Interferon-gamma;Antiviral activity
期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.534; 五年影响因子:2.382 )
ISSN:
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收录情况: SCI
摘要: Minks (Neovison vison) farming is under a threat of a variety of viral infections with increasingly growing number of breeding in Northeastern and Western China. While interferon is effective in controlling viral infection, IFN among different species rarely share high homology enough to provide cross protective effect on inhibition of virus replication. We cloned, sequenced, phlogenetically analyzed and expressed the miIFN-gamma gene in prokaryotic and eukaiyotic cells. The anti-vesicular stomatitis virus (VSV) activity of miIFN-gamma protein was tested in MDCK cells using in vitro cytopathic inhibition assay. The recombinant miIFN-gamma could inhibit VSV replication in MDCK cells, which was confirmed by that pre-incubation of rabbit anti-miIFN-gamma antibodies with miIFN-gamma abrogated the miIFN-gamma protective effect. Our findings implicated that the milFN-gamma gene may be a potential counter measure against viral infection in the mink farming. (C) 2015 Published by Elsevier Ltd.
分类号: S85
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