Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea

文献类型： 外文期刊

第一作者： Zhang, Dong Ling

作者： Zhang, Dong Ling;Chen, Jian;Wang, Zhi Yong;Yu, Da Hui

作者机构：

关键词： BCA2;Larimichthys crocea;Immune response;In-situ hybridization;E3 ubiquitin ligase

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： BCA2, as an E3 ubiquitin ligase, is an important anti-virus immune factor in mammals. Up to date, there are not any related reports on BCA2 protein in fishes yet. In the present investigation, BCA2 in large yellow croaker Larimichthys crocea (named as LcBCA2) was identified and characterized. The full-length cDNA of LcBCA2 was 1571 bp, including an ORF of 888 bp encoding a polypeptide of 295 amino acids. The putative LcBCA2 protein contained a RING-H2 motif at C terminal. The LcBCA2 transcripts were broadly distributed in all detected tissues, with high expression in muscle, moderate in blood, skin, heart, liver and spleen, weak in other tissue as indicated by qPCR analysis. Significant increases were observed in skin, gill and spleen after infection of Cryptocaryon irritans, and in spleen and head-kidney after inactivated Vibrio. parahaemolyticus, LPS and Poly I:C stimulations. Tissue localization by in-situ hybridization showed that LcBCA2 mainly expressed in the spleen of the fish in the test group. Our findings showed that LcBCA2 inclined to sharply increase in immune organs, especially in head-kidney after bacterial and viral stimulations, while in locations (skin and gill) of parasites infections, suggesting that BCA2 may play an important role in fish defense against bacteria, virus and parasites infections, but the immune mechanisms is are different. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]Characterization of E3 ubiquitin ligase neuregulin receptor degradation protein-1 (Nrdp1) in the large yellow croaker (Larimichthys crocea) and its immune responses to Cryptocaryon irritans. Zhang, Dong Ling,Han, Fang,Xiao, Shi Jun,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui,Li, Ming Yun.

[2]Introgression of a novel Thinopyrum intermedium St-chromosome-specific HMW-GS gene into wheat. Liu, Cheng,Li, Cheng-Hui,Zhao, Jin-Mei,Zhou, Li,Dai, Gang,Yang, Zu-Jun,Yang, En-Nian. 2013

[3]Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th ponticum. Li, Hongjie,Conner, R. L.,Murray, T. D..

[4]Pathological changes in masked palm civets experimentally infected by severe acute respiratory syndrome (SARS) coronavirus. Xiao, Y.,Meng, Q.,Yin, X.,Guan, Y.,Liu, Y.,Li, C.,Wang, M.,Liu, G.,Tong, T.,Kong, X.,Wu, D.,Xiao, Y.,Meng, Q.,Liu, G.,Tong, T.,Kong, X.,Wu, D.,Wang, L. -F.. 2008

[5]Loss of chromosomes 2R and 5RS in octoploid triticale selected for agronomic traits. Cheng, ZJ,Murata, M.

[6]Co-expression of march5b and tlr7 in large yellow croaker Larimichthys crocea in response to Cryptocaryon irritans infection. Zhang, D. L.,Chen, J.,Wang, Z. Y.,Yu, D. H.,Chen, C..

[7]The Arabidopsis U-box/ARM repeat E3 ligase AtPUB4 influences growth and degeneration of tapetal cells, and its mutation leads to conditional male sterility. Wang, Hai,Jiang, Tiantian,Xia, Yiji,Wang, Hai,Berg, Howard,Xia, Yiji,Lu, Yuqing,Li, Cong. 2013

[8]A zebrafish (Danio rerio) bloodthirsty member 20 with E3 ubiquitin ligase activity involved in immune response against bacterial infection. Zhang, Xinshang,Zhao, Heng,Chen, Yeyu,Luo, Huiying,Yang, Peilong,Yao, Bin.

[9]Characterization and biological function analysis of the trim3a gene from zebrafish (Danio rerio). Zhang, Xinshang,Zhao, Heng,Chen, Yeyu,Meng, Kun,Yang, Peilong,Wang, Yaru,Wang, Guozeng,Yao, Bin,Liu, Chao. 2012

[10]Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Liu, Bin,Zuo, Zecheng,Liu, Hongtao,Lin, Chentao,Liu, Bin,Zuo, Zecheng,Liu, Xuanming. 2011

[11]Anti-parasitic effects of Leptomycin B isolated from Streptomyces sp CJK17 on marine fish ciliate Cryptocaryon irritans. Yin, Fei,Sun, Peng,Tang, Baojun,Gong, Hui,Ke, Qiaozhen,Li, Anxing.

[12]Mass mortality associated with a viral-induced anaemia in cage-reared large yellow croaker, Larimichthys crocea (Richardson). Xu, W. J.,Shi, H.,Xie, J. J.,Wang, G. S.,Qin, Z. D.,Zhou, Y.,Liu, X. L.,Asim, M.,Lin, L.,Jiang, N.,Zeng, L. B.,Wang, W. M.,Lin, L.,Lin, L.,Lin, L..

[13]Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker (Larimichthys crocea) to ciliate ectoparasite Cryptocaryon irritans infection. Yin, Fei,Gao, Quanxin,Tang, Baojun,Sun, Peng,Han, Kunhuang,Huang, Weiqing,Han, Kunhuang,Huang, Weiqing.

[14]Characterisation and expression analysis of two terminal complement components: C7 and C9 from large yellow croaker, Larimichthys crocea. Guo, Baoying,Wu, Changwen,Lv, Zhenming,Liu, Changlin.

[15]Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea. Zhang, Dong Ling,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui,Fan, Sigang.

[16]Molecular cloning and expression analysis of scd1 gene from large yellow croaker Larimichthys crocea under cold stress. Xu, Hao,Zhang, Dong Ling,Lv, Chang Huan,Luo, Hui Yu,Wang, Zhi Yong,Yu, Da Hui.

[17]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[18]Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D, L-lactic acid) nanospheres. Zheng, Sisi,Luo, Li,Liu, Zhenguang,Bo, Ruonan,Hu, Yuanliang,Liu, Jiaguo,Wang, Deyun,Qin, Tao,Huang, Yifan,Wang, Deyun,Lu, Yu. 2018

[19]Plasmid containing CpG motifs enhances the efficacy of porcine reproductive and respiratory syndrome live attenuated vaccine. Guo, Xiaoyu,Hou, Shaohua,Zhu, Hongfei,Guo, Xiaoyu,Sanchez-Vizcaino, J. M.,Guo, Xiaoyu,Sanchez-Vizcaino, J. M.,Zhang, Quan,Zhai, Guoqin. 2011

[20]A genome-wide association study identifies major loci affecting the immune response against infectious bronchitis virus in chicken. Luo, Chenglong,Qu, Hao,Ma, Jie,Wang, Jie,Shu, Dingming,Hu, Xiaoxiang,Li, Ning,Luo, Chenglong,Qu, Hao,Ma, Jie,Wang, Jie,Shu, Dingming. 2014

作者其他论文 更多>>

微信小程序