Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis

文献类型： 外文期刊

第一作者： Chen, Fanjie

作者： Chen, Fanjie;Hu, Han;Li, Zhonghua;Huang, Jiacheng;Cai, Xuwang;Wang, Chunmei;He, Qigai;Cao, Jiyue;Wang, Chunmei

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关键词： Cecropin B;Haemophilus parasuis;Periplasmic substrate-binding protein

期刊名称：JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：0.803； 五年影响因子：1.609 ）

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收录情况： SCI

摘要： Cecropin B (CB) is a very effective natural antimicrobial peptide that has shown great potential for future antimicrobial drug development. HAPS_2096 is a Haemophilus parasuis gene that encodes the periplasmic substrate-binding protein of an ATP-binding cassette-type amino acid transporter. In this research, we constructed and verified an HAPS_2096 deletion mutant and a complementary HAPS_ 2096 mutant of H. parasuis JS0135. A bactericidal assay revealed that the HAPS_ 2096 deletion mutant was significantly more sensitive than the wild-type strain to 0.25-0.5 mu g/ml CB. However, the gene complementation alleviated the CB sensitivity of the mutant. Immunoelectron microscopy observation following a 30-min treatment with a sublethal concentration of CB (0.25 mu g/ml) revealed more extensive morphological damage in the mutant strain than in the wild-type strain. Hence, our results suggest that the HAPS_2096 gene contributes to H. parasuis resistance to CB. (C) 2015 S. Karger AG, Basel

分类号： Q93`Q81

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