A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae

文献类型： 外文期刊

第一作者： Qi, Xiliang

作者： Qi, Xiliang;Qi, Juncang;Qi, Xiliang;Su, Xiaofeng;Guo, Huiming;Cheng, Hongmei

作者机构：

关键词： DNA end-joining repair;Verticillium dahliae;Homologous recombination;Delta Vdku70 strain;Targeted gene replacement

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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收录情况： SCI

摘要： To overcome the challenges met with gene deletion in the plant pathogen Verticillium dahliae, a mutant strain with impaired non-homologous end joining DNA repair was generated to improve targeted gene replacement frequencies. A V. dahliae 991 Delta Vdku70 null mutant strain was generated using Agrobacterium tumefaciens-mediated transformation. Despite having impaired non-homologous end joining DNA repair function, the Delta Vdku70 strain exhibited normal growth, reproduction capability, and pathogenicity when compared with the wild-type strain. When the Delta Vdku70 strain was used to delete 2-oxoglutarate dehydrogenase E2, ferric reductase transmembrane component 3 precursor, and ferric reductase transmembrane component 6 genes, gene replacement frequencies ranged between 22.8 and 34.7 % compared with 0.3 and 0.5 % in the wild-type strain. The Delta Vdku70 strain will be a valuable tool to generate deletion strains when studying factors that underlie virulence and pathogenesis in this filamentous fungus.

分类号： Q93

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