Real-time PCR as a diagnostic tool for evaluating the resistance of Chinese cabbage cultivars to Verticillium wilt

文献类型: 外文期刊

第一作者: Yu, Shuancang

作者: Yu, Shuancang;Su, Tongbing;Chen, Juan;Wang, Weihong;Zhang, Fenglan;Yu, Yangjun;Zhang, Deshuang;Zhao, Xiuyun;Lu, Guixiang;Yu, Shuancang;Su, Tongbing;Chen, Juan;Wang, Weihong;Zhang, Fenglan;Yu, Yangjun;Zhang, Deshuang;Zhao, Xiuyun;Lu, Guixiang

作者机构:

关键词: Brassica rapa subsp pekinensis;Verticillium wilt;V. longisporum;Real-time PCR

期刊名称:EUROPEAN JOURNAL OF PLANT PATHOLOGY ( 影响因子:1.907; 五年影响因子:2.022 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Verticillium wilt disease has become increasingly serious in many regions in China over the past few years. In this study, the widespread pathogen was identified as Verticillium longisporum (V. longisporum) by mt-SSU rDNA and cytochrome b sequence analysis. By using the primer pair HW1-F/HW1-R, which was designed based on the V. longisporum-specific rDNA-ITS sequence, a real-time PCR assay was developed for rapid and specific detection of the pathogen. The real-time PCR assay enabled sensitive quantification of V. longisporum in artificial and field-infected seedlings. This study provides a method for large-scale screening of Verticillium wilt-resistant resources for Chinese cabbage breeding.

分类号: S432.1

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