Immunogenicity of different recombinant rabbit hemorrhagic disease virus-like particles carrying CD8+T cell epitope from chicken ovalbumin (OVA)
文献类型: 外文期刊
第一作者: Chen, Mengmeng
作者: Chen, Mengmeng;Song, Yanhua;Fan, Zhiyu;Hu, Bo;Xue, Jiabin;Wei, Houjun;Wang, Fang;Chen, Mengmeng;Jiang, Ping
作者机构:
关键词: Rabbit hemorrhagic disease virus;Capsid protein (VP60);T cell epitope;Virus-like particles;Immunogenicity
期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: To explore the capacity and immunogenicity of virus-like particles (VLPs) of rabbit hemorrhagic disease virus (RHDV) accommodating foreign amino acid sequences, integrations were performed at the following four locations of the structural protein VP60 of RHDV using the OVA(257-264) CD8+ T cell epitope (SIINFEKL): (1) inserting at the N-terminus of the VP60 protein (N1); (2) replacing amino acid positions 2-14 of the VP60 protein (N2); (3) replacing amino acid positions 196-207 of the VP60 protein (I1); and (4) replacing amino acid positions 217-228 of the VP60 protein (I2). The recombinant proteins were expressed by baculovirus expression system. The ability to form RHDV-like particles was confirmed by electron microscopy. The immunogenicity of the four recombinant proteins (N1, N2, I1 and I2) was evaluated in mice without any adjuvants. The results indicated that the four recombinant proteins (N1, N2, I1 and I2) could assemble into VLPs. All of the recombinant proteins could induce a specific immune response. Recombinant proteins I1 and I2 were able to elicit both high levels of IFN-gamma secretion and anti-VP60 specific immune responses in the murine model. The levels of the VP60-specific IgG antibody in groups I1 and I2 displayed higher optical density (OD) values than those of groups N1 and N2 (P<0.001, P<0.001). The number of IFN-gamma-producing splenocytes in mice that were immunized with recombinant proteins I1 and I2 was also significantly greater compared with mice that were immunized with recombinant proteins N1 and N2 (P<0.01). All of these above mentioned results might be beneficial to the establishment of the RHDV-VLPs display system. (c) 2014 Published by Elsevier B.V.
分类号: R37
- 相关文献
作者其他论文 更多>>
-
A ratiometric fluorescent probe for the determination of quinolone antibiotics in milk based on N and S co-doped carbon quantum dots
作者:Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Han, Jiajun;Gong, Wenwen
关键词:Carbon quantum dot; Ratiometric fluorescence probe; Moxifloxacin; Gatifloxacin; Ofloxacin; Milk
-
Water-soluble stimuli-responsive supramolecular nanoagrochemicals based on macrocycle compounds
作者:Song, Ya-Hui;Bian, Qiang;Yang, Yi-Hang;Zhang, Ying-Ming;Liu, Yu;Wang, Fang;Liu, Jinpeng
关键词:Supramolecular assemblies; Host-guest complexation; Macrocycles; Stimuli-responsiveness; Nanoagrochemicals
-
Rapid detection and spectroscopic feature analysis of mineral content in camel milk using fourier-transform mid-infrared spectroscopy and traditional machine learning algorithms
作者:Li, Yongqing;Fan, Yikai;Gao, Jingyi;Liu, Li;Wang, Haitong;Chu, Chu;Yang, Zhuo;Yang, Guochang;Wen, Peipei;Wang, Dongwei;Zhang, Shujun;Li, Yongqing;Liu, Li;Cao, Lijun;Hu, Bo;Abula, Zunongjiang;Xieermaola, Yeerlan;Zheng, Wenxin
关键词:Camel milk; Minerals; FT-MIRS; Machine learning; Detection method
-
High-precise determination of the drought and cold resistance of forage seeds using terahertz time-domain spectroscopy
作者:Wang, Fang;Xiao, Ziwei;Liu, Zilong;Zhang, Chunhong;Yin, Panpan;Liu, Lemeng;Xiang, Wenfeng
关键词:Terahertz time domain spectroscopy; Cold and drought resistance; Forage seeds; Multilayer structure propagation model
-
Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice
作者:Luo, Biao;Rao, Liqun;Wang, Qiming;Luo, Biao;Zhang, Xianwen;Wang, Fang;Wang, Yan;Zhang, Xianwen;Wu, Wei;Lin, Chaoyang
关键词:5-Enolpyruvyl-shikimate-3-phosphate synthase (EPSPS); DAS-ELISA; OsmEPSPS detection; Glyphosate-tolerant rice; Glyphosate-tolerant rice
-
Inhibition of transcriptional regulation of detoxification genes contributes to insecticide resistance management in Spodoptera exigua
作者:Hu, Bo;Deng, Yuping;Lu, Tao;Hu, Bo;Deng, Yuping;Lu, Tao;Ren, Miaomiao;Liu, Kuitun;Rao, Cong;Guo, Hailiang;Su, Jianya
关键词:
-
Emergence of Canine Parvovirus Type 2a and Canine Enteric Coronavirus in Diarrheal Outbreaks Among Farmed Raccoon Dogs in China
作者:Lu, Rongguang;Yang, Wei;Qian, Tingli;Li, Guobao;Lu, Rongguang;Cao, Haixu;Fu, Jianhai;Zhang, Chengqi;Hu, Bo;Xu, Liwen;Li, Shuangshuang;Zhao, Guanyu;Bai, Xue;Feng, Na;Shi, Qiumei;Zang, Deyue
关键词:epidemiology; evolution; mixed infections; molecular characteristics; receptor interaction