The Zn(II)2Cys6 putative transcription factor is involved in the regulation of leucinostatin production and pathogenicity of the nematophagous fungus Paecilomyces lilacinus

文献类型： 外文期刊

第一作者： Yang, Fan

作者： Yang, Fan;Abdelnabby, Hazem;Xiao, Yannong;Yang, Fan;Abdelnabby, Hazem

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关键词： gene overexpression;knockout;leucinostatins;Meloidogyne incognita;Paecilomyces lilacinus

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）

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收录情况： SCI

摘要： Zn(II)2Cys6 transcription factor genes encode transcription regulators that manage the infection potential and production of secondary metabolites such as toxins in fungi. In this study, a gene named rolP that encodes a putative Zn(II)2Cys6 transcription factor regulating leucinostatin production in the filamentous fungus Paecilomyces lilacinus was characterized by a gene knockout approach. The deduced proprotein consists of 705 amino acids and is highly homologous to the Zn(II)2Cys6 transcription factor of the entomopathogenic fungus Metarhizium brunneum. Predictive analysis of the secondary structure of the proportion showed that it has two domains. Paecilomyces lilacinus can produce nematotoxins leucinostatins A and B. Deletion of rolP from the P. lilacinus wild-type strain Pl36-1 leads to the absence of leucinostatin A, while a large increase in the leucinostatin A level was achieved in the rolP overexpression strain Ov-Pl36-1. During the process of nematode infection, rolP showed high expression levels at 48-72 h and peaked at 48 h. Bioassay tests confirmed the requirement of rolP for leucinostatin production. However, the root-knot second-stage juvenile-toxicity was minimized to 35.3% in rolP; toxicity was 97.2% with Ov-Pl36-1 compared with 68.4% with the wild type strain Pl36-1. Interestingly, neither knockout nor overexpression of the rolP gene affected the growth or sporulation of P. lilacinus. Additionally, fungal nutrition and acidic media might stimulate the rolP activity of the wild type strain against nematodes. These findings suggested that the rolP gene is required for the induction and production of leucinostatins and is considered a leucinostatin regulatory gene in P. lilacinus. ResumeLes genes du facteur de transcription Zn(II)2Cys6 encodent les regulateurs de transcription qui gerent le potentiel d'infection et la production de metabolites secondaires, comme des toxines, chez les champignons. Dans cette etude, un gene nomme rolP, qui encode un facteur de transcription putatif Zn(II)2Cys6 regulant la production de leucinostatine chez le champignon filamenteux Paecilomyces lilacinus, a ete caracterise grace a un gene inactive. La proproteine qui en a ete deduite consiste en 705 acides amines et est fortement homologue au facteur de transcription Zn(II)2Cys6 du champignon entomopathogene Metarhizium brunneum. L'analyse previsionnelle de la structure secondaire de la proportion a montre qu'elle possede deux domaines. P. lilacinus peut produire les leucinostatines A et B, des nematotoxines. La deletion du gene rolP de la souche sauvage Pl36-1 de P. lilacinus provoque l'absence de leucinostatine A, tandis que l'on a note une forte augmentation du taux de leucinostatine A lors de la surexpression du gene rolP de la souche OvPl36-1. Durant l'infection causee par le nematode, le gene rolP a affiche des taux eleves d'expression de 48 a 72h, et a atteint son plus haut taux a 48h. Des biotests ont confirme que le gene rolP etait necessaire a la production de leucinostatine. Toutefois, la toxicite induite par le gene rolP chez les larves de deuxieme stade du nematode cecidogene etait reduite a 35.3%; la toxicite chez la souche Ov-Pl36-1 etait de 97.2%, comparativement a 68.4% chez la souche sauvage Pl36-1. Chose interessante, ni l'inactivation ni la surexpression du gene rolP n'ont influence la croissance ou la sporulation de P. lilacinus. De plus, chez la souche sauvage, le mode d'alimentation du champignon et l'acidite du milieu peuvent stimuler l'activite du gene rolP contre les nematodes.

分类号： S4

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