Sulforaphane protects human umbilical vein cells against lipotoxicity by stimulating autophagy via an AMPK-mediated pathway

文献类型： 外文期刊

第一作者： Wang, Ning

作者： Wang, Ning;Zhao, Juyang;Liu, Qian;Kong, Baohua;Wang, Ning;Liu, Qian;Kong, Baohua;Diao, Xinping

作者机构：

关键词： Sulforaphane;Saturated fatty acid;Autophagy;Lipotoxicity;Endothelial cell

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：4.451； 五年影响因子：4.907 ）

ISSN：

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收录情况： SCI

摘要： The protective role of sulforaphane (SUN) against saturated fatty acid (SFA)-induced lipotoxicity in human umbilical vein endothelium cell was determined. SUN protected endothelium cells against SPA-induced cell death. This protective activity was Nrf2-independent because silencing Nrf2 did not block the protective action of SFN. Subsequent studies revealed that SFN-induced autophagy contributed to the protective role. Incubation of the cells with autophagy inhibitors significantly blocked the protection by SUN. In addition, SFN significantly enhanced the formation of LC3-II and increased autophagic flux. Mechanistic study revealed that SFN significantly stimulated the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and reduced the phosphorylation of the mammalian target of rapamycin (mTOR). Moreover, knocking-down AMPK significantly blocked SUN-induced autophagy and inhibited its protective role against lipotoxicity. This finding provides novel data demonstrating that SUN protects endothelial cells from SPA-induced lipotoxicity through activating autophagy via an AMPK/mTOR involved pathway. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2

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