DNA methylation and NF-Y regulate Piwil1 expression during chicken spermatogenesis
文献类型: 外文期刊
第一作者: Chang, Guobin
作者: Chang, Guobin;Chen, Rong;Xu, Lu;Ma, Teng;Wang, Hongzhi;Chen, Jing;Zhang, Yang;Li, Zhiteng;Wan, Fang;Guo, Xiaomin;Xu, Qi;Zhao, Wenming;Chen, Guohong;Chen, Rong
作者机构:
关键词: Chicken;DNA methylation;Piwil1;Spermatogenesis
期刊名称:ANIMAL REPRODUCTION SCIENCE ( 影响因子:2.145; 五年影响因子:2.281 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The P-element induced wimpy testis (Piwi) protein family, a subfamily of the Argonaute protein family, is involved in gene silencing and shows specific expression in spermatogenic cells. To reveal the transcriptional regulatory mechanisms of Piwil1 in chickens, we cloned sequences of the chicken Piwil1 promoter region and performed luciferase reporter and electrophoretic mobility shift assays to analyze the transcriptional activity and identify important transcriptional regulatory elements. The results showed that the region from -90 to -43 in the 5'-flanking region of Piwil1 contains a transcriptional regulatory CCAAT box that was necessary for the transcriptional activity of the Piwil1 promoter. Moreover, the transcription factor nuclear factor Y (NF-Y) was bound to the Piwil1 promoter CCAAT box specifically in germ cells. In addition, bisulfite sequencing to determine the methylation profile of the Piwil1 promoter CpG island in different spermatogenic and non-germ cell populations was performed. Compared with germ cells, non-germ cells showed increased methylation of the promoter region containing the CCAAT box, loss of NF-Y binding, and silencing of the Piwil1 locus. It is demonstrated that the specific expression of Piwil1 in chicken germ cells is regulated by the transcription factor NF-Y and differential CpG island methylation. (C) 2015 Elsevier B.V. All rights reserved.
分类号: Q95
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