Chronic stress effects of high doses of vitamin D-3 on Megalobrama amblycephala

文献类型: 外文期刊

第一作者: Miao, Ling-Hong

作者: Miao, Ling-Hong;Xie, Jun;Ge, Xian-Ping;Wang, Ke-Bao;Zhu, Jian;Liu, Bo;Zhou, Qun-Lan;Miao, Ling-Hong;Xie, Jun;Ge, Xian-Ping;Zhu, Jian;Liu, Bo;Ren, Ming-Chun;Zhou, Qun-Lan;Pan, Liang-Kun

作者机构:

关键词: Megalobrama amblycephala;Vitamin D-3;Chronic stress;Immunity;Tissue structure

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

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收录情况: SCI

摘要: Dietary vitamin D-3 plays an important role in the growth of aquatic animals, but long-term excessive feeding has potential hazards. In this study, Megalobrama amblycephala specimens were fed different experimental diets with 2000 IU/kg or 200,000 IU/kg of vitamin D-3 for 90 days, in order to evaluate chronic stress effects of high doses of vitamin D-3 on growth, immunity, and structural damage to enterohepatic tissues. The results showed that high doses of vitamin D-3 did not have a significant influence on the growth performance of M. amblycephala (P > 0.05), but it significantly reduced the survival rate after infection by Aeromonas hydrophila (P < 0.05). Serum albumin, alkaline phosphatase, and insulin levels, as well as hepatic total antioxidant capacity, were also significantly reduced (P < 0.05). Serum cortisol levels and hepatic heat stress protein 70 expression in M. amblycephala showed that high doses of vitamin D-3 significantly inhibit the anti-stress ability of M. amblycephala (P < 0.05). Paraffin tissue sections and electron microscopy showed that high doses of vitamin D-3 could cause different degrees of structural damage to enterohepatic tissues of M. amblycephala. Our results indicate that, although M. amblycephala can tolerate high doses of dietary vitamin D-3 over a long period, its glycolipid metabolism, immune function, anti-stress function, and resistance to pathogenic infections are adversely affected. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: S9

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