Effects of temperature and dietary protein on gene expression of Hsp70 and Wap65 and immunity of juvenile mirror carp (Cyprinus carpio)

文献类型： 外文期刊

第一作者： Huang, Jin-feng

作者： Huang, Jin-feng;Xu, Qi-you;Chang, Yu-mei;Huang, Jin-feng

作者机构：

关键词： mirror carp;immunity;Hsp70;Wap65;temperature;dietary protein level

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

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收录情况： SCI

摘要： Juvenile mirror carp were fed diets containing 303.4, 321.7, 341.2, 361.0 and 379.1gkg(-1) proteins, respectively, and reared at different water temperatures (18, 23 and 28 degrees C) for 60days. Gene expression of heat shock protein gene (Hsp70) and the warm temperature acclimation-related 65 kDa protein gene (Wap65), immunity and antioxidant status in the carp were investigated. Results indicated that the contents of serum complement 3 (C3), complement 4 (C4) and immunoglobulin M (IgM), as well as activities of liver superoxide dismutase (SOD) and lysozyme (LSZ) were significantly enhanced with increasing dietary protein (P<0.05), while content of malondiadehyde (MDA) decreased. Gene expression level of Wap65 in the liver significantly increased with dietary protein, while gene expression of Hsp70 decreased. The contents of C3, C4 and IgM, the activities of SOD and LSZ and gene expression level of Wap65 in the liver significantly increased with temperature. These results suggest that: Serum immune parameter, antioxidant enzymes and Hsp70 and Wap65 expression interact in fish to improve ability to adapt to the environment; and the optimal conditions for the immunity of carp are 348.1354.5gkg(-1) protein at 18 degrees C, 352.3364.9gkg(-1) at 23 degrees C and 360.2364.3gkg(-1) at 28 degrees C, and the optimum temperature for carp is 23 degrees C.

分类号： S9

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