Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus

文献类型： 外文期刊

第一作者： Chen, Yuming

作者： Chen, Yuming;Lu, Zhen;Zhang, Lizhou;Gao, Li;Wang, Nian;Gao, Xiang;Wang, Yongqiang;Li, Kai;Gao, Yulong;Cui, Hongyu;Gao, Honglei;Liu, Changjun;Zhang, Yanping;Qi, Xiaole;Wang, Xiaomei;Wang, Xiaomei

作者机构：

关键词： Infectious bursal disease virus;Ribosomal protein L4;VP3;Interaction;Replication

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： VP3 protein is a structural protein which plays important roles in the virus assembly and the inhibition of antiviral innate immunity of infectious bursal disease virus (IBDV). To explore the potential roles of VP3 in the interplay of IBDV with the host cell, an immunoprecipitation (IP)-coupled mass spectra (MS) screening was performed and the host cellular ribosomal protein L4 (RPL4) was identified as a putative interacting partner of VP3 protein. The interaction of RPL4 with VP3 was further confirmed by co-immunoprecipitation (co-IP) and their colocalization in DF1 cells were observed by confocal microscopy. In addition, knockdown of RPL4 in DF1 cells resulted in reductions of the viral protein pVP2 expression and the virus titers, which reveals a significant role of RPL4 in IBDV replication. Taken together, we indicated for the first time that ribosomal protein L4 (RPL4) was an interacting partner of VP3 and involved in the modulation of IBDV replication. The present study contributes to further understanding the pathogenic mechanism of IBDV. (C) 2015 Elsevier B.V. All rights reserved.

分类号： R37

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