Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum

文献类型: 外文期刊

第一作者: Cao, Xiaodan

作者: Cao, Xiaodan;Fu, Zhiqiang;Han, Qian;Lu, Ke;Li, Hao;Zhu, Chuangang;Hong, Yang;Lin, Jiaojiao;Lin, Jiaojiao;Zhang, Min;Han, Yanhui

作者机构:

关键词: Schistosoma japonicum;Schistosomula;Proteome;Excretory/secretory proteins;Thioredoxin peroxidase;Macrophages

期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Schistosomiasis remains a serious public health problem, with 200 million people infected and 779 million people at risk worldwide. The schistosomulum is the early stage of the complex lifecycle of Schistosoma japonicum in their vertebrate hosts, and is the main target of vaccine-induced protective immunity. Excretory/secretory (ES) proteins play a major role in host parasite interactions and ES protein compositions of schistosomula of S. japonicum have not been characterized to date. In the present study, the proteome of ES proteins from 14 day schistosomula of S. japonicum was analyzed by liquid chromatography/tandem mass spectrometry and 713 unique proteins were finally identified. Gene ontology and pathway analysis revealed that identified proteins were mainly involved in carbohydrate metabolism, degradation, response to stimulus, oxidation-reduction, biological regulation and binding. Flow cytometry analysis demonstrated that thioredoxin peroxidase identified in this study had the effect on inhibiting MHCII and CD86 expression on LPS-activated macrophages. The present study provides insight into the growth and development of the schistosome in the final host and valuable information for screening vaccine candidates for schistosomiasis. (C) 2015 Elsevier B.V. All rights reserved.

分类号: Q51

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