Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine

文献类型： 外文期刊

第一作者： Li, Xiujie

作者： Li, Xiujie;Sun, Yaning;Yang, Suzhen;Wang, Yinbiao;Yang, Jifei;Liu, Yunchao;Jin, Qianyue;Li, Xuewu;Guo, Chengliu;Zhang, Gaiping;Zhang, Gaiping;Li, Xiujie;Yang, Suzhen;Zhang, Gaiping

作者机构：

关键词： Enzyme-linked immunosorbent assay;immunochromatographic strip;pseudorabies virus;swine

期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： An immunochromatographic strip was developed for the serological detection of pseudorabies virus (PRV) in swine. In the strip, the expressed protein of gB, one of the glycoproteins of PRV, labeled with colloidal gold, was used as the detector; staphylococcal protein A and swine anti-pseudorabies virus antibody were blotted on nitrocellulose membrane for the test and control lines, respectively. The specificity of the strip was 98.1%, and the sensitivity of the strip with reference anti-PRV serum was 96.0%. Swine serum samples (296) were collected to evaluate the characteristics of the strip in comparison with an existing commercial kit. The agreement was 93.6%. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents, or equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.

分类号： S85

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