Cytotoxic effects and apoptosis induction of enrofloxacin in hepatic cell line of grass carp (Ctenopharyngodon idellus)

文献类型： 外文期刊

第一作者： Liu, Bo

作者： Liu, Bo;Ge, Xianping;Xie, Jun;Xu, Pao;Liu, Bo;Cui, Yanting;Ge, Xianping;Xie, Jun;Xu, Pao;Brown, Paul B.

作者机构：

关键词： Enrofloxacin;Cytotoxicity;Apoptosis;ROS;Grass carp;Hepatic cell

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： We determined the effect of enrofloxacin on the lactate dehydrogenase (LDH) release, reactive oxygen species (ROS), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde (MDA), mitochondria membrane potential (Delta Psi m) and apoptosis in the hepatic cell line of grass carp (Ctenopharyngodon idellus). Cultured cells were treated with different concentrations of enrofloxacin (12.5-200 ug/mL) for 24 h. We found that the cytotoxic effect of enrofloxacin was mediated by apoptosis, and that this apoptosis occurred in a dose-dependent manner. The doses of 50,100 and 200 mu g/mL enrofloxacin increased the LDH release and MDA concentration, induced cell apoptosis and reduced the Delta Psi m compared to the control. The highest dose of 200 ug/mL enrofloxacin also significantly induced apoptosis accompanied by Delta Psi m disruption and ROS generation and significantly reduced T-AOC and increased MDA concentration compared to the control. Our results suggest that the dose of 200 ug/mL enrofloxacin exerts its cytotoxic effect and produced ROS via apoptosis by affecting the mitochondria of the hepatic cells of grass carp. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： S9

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