Analysis of genetic relations and evaluation of medicinal value among Hedera plants in China by sequence related amplified polymorphism markers and high performance liquid chromatography detection

文献类型： 外文期刊

第一作者： Li, Fang

作者： Li, Fang;Cao, Xiong-jun;Ruan, Qin-mei;Zhong, Xiao-hong;Li, Fang;Cao, Xiong-jun

作者机构：

关键词： Hedera plant;SRAP molecular marker;Cluster analysis;HPLC detection;Medicinal value

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hedera plants are ornamental vines which are regarded as traditional medicinal plants. These plants are widely cultivated worldwide; moreover, new species and varieties have been discovered. For the first time, molecular markers based on sequence-related amplified polymorphism (SRAP) were used in this study to classify species and to analyse the genetic relationship of 21 Hedera plant varieties which are commonly cultivated in China. We also employed high-performance liquid chromatography (HPLC) to evaluate the medicinal value of these plants by measuring their hederacoside C contents. Results of SRAP -PCR and cluster analyses show that the SRAP molecular markers can successfully be applied in Hedera plants and are available. In addition, HPLC detection results indicate that the Chinese wild species in group I contain less than 30 mg/g hederacoside C and do not comply with the European Pharmacopoeia standards. By contrast, groups II and III exhibit high medicinal value and accord with these standards. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： Q5

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