Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities

文献类型： 外文期刊

第一作者： Fu, Bo

作者： Fu, Bo;Liu, Di;Ma, Hong;Zhang, Dong-Jie;Guo, Zhen-Hua;Fu, Bo;Ma, Jian-Zhang;An, Tie-Zhu;Ren, Liang;Liu, Di;Yang, Xiu-Qin;Guo, Yun-Yun;Zhu, Meng;Bai, Jing

作者机构：

关键词： Porcine Oocytes;Brilliant Cresyl Blue;Embryonic Development;Subcellular Characteristics

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB-oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.

分类号： Q95

  • 相关文献

[1]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[2]POSITIVE EFFECT OF APOPTOTIC INHIBITOR Z-VAD-FMK ON VITRIFIED-THAWED PORCINE MII STAGE OOCYTES. Niu, Yingfang,Dai, Jianjun,Chen, Yaning,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[3]Comparison of gene expression patterns between porcine cumulus-oocyte complexes and naked oocytes. Zhu, G. Y.,Feng, S. T.,Li, J. T.,Mu, Y. L.,Pan, D. K.,Guo, B. R.. 2007

[4]MC1568 Enhances Histone Acetylation During Oocyte Meiosis and Improves Development of Somatic Cell Nuclear Transfer Embryos in Pig. Wang, Huili,Meng, Chunhua,Zhang, Jun,Li, Yinxia,Qian, Yong,Xing, Guangdong,Cao, Shaoxian,Cui, Wei,Zhao, Dongmin. 2018

[5]A new tool for in vitro culture of porcine eggs. Zheng, Xin-Min,Qiao, Xian-Feng,Wei, Qing-Xin,Xiao, Hong-Wei,Liu, Xi-Mei,Li, Li,Hua, Zai-Dong,Xu, Hou-Qiang. 2011

[6]Factors affecting the survival, fertilization, and embryonic development of mouse oocytes after vitrification using glass capillaries. Tan, Xiuwen,Song, Enliang,Liu, Xiaomu,You, Wei,Wan, Fachun.

[7]Histology and ultrastructure of the thymus during development in tilapia, Oreochromis niloticus. Cao, Jianmeng,Chen, Qiong,Lu, Maixin,Hu, Xinxin,Wang, Miao,Chen, Qiong,Hu, Xinxin.

[8]Comparison of the developmental competence and quality of bovine embryos obtained by in vitro fertilization with sex-sorted and unsorted semen from seven bulls. Liu, Xia,Hu, Tingxi,Sun, Weijun,Hao, Haisheng,Liu, Yan,Zhao, Xueming,Zhu, Huabin,Du, Weihua,Hu, Tingxi.

[9]Injection of frozen-thawed porcine first polar bodies into enucleated oocytes results in fertilization and embryonic development. Wang, G. J.,Yu, J. N.,Xu, X. B.,Fan, B. Q.,Tan, X. D.,Zhou, X. L..

[10]Fecundity and Brood Loss of Sand Shrimp, Crangon uritai (Decapoda: Crangonidae). Li, Hui Yu,Hong, Sung Yun,Jin, Zong Hua.

[11]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[12]Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua,Ouyang, Hongjia,Wang, Zhijun,Chen, Xiaolan,Yu, Jiao,Li, Zhenhui,Nie, Qinghua. 2017

[13]Transcriptome analysis of gene expression patterns during embryonic development in golden cuttlefish (Sepia esculenta). Bian, Li,Liu, Changlin,Chen, Siqing,Zhao, Fazhen,Ge, Jianlong,Tan, Jie. 2018

[14]BmARM-Like Protein from Silkworm, Bombyx mori (Lepidoptera) is Putatively Involved in Response against BmNPV Infection. Wang, Xue-yang,Zhang, Shang-zhi,Yu, Dong,Ma, Yan,Fei, Dong-qiong,Yu, Hai-zhong,Xu, Jia-ping,Liu, Ming-hui. 2017

[15]A study on the effects of Alexandriurn minuturn and Gymnodiniurn on the embryonic development of Sparus macrocephalus. Li, Lei,Shen, Xinqiang,Wang, Yunlong,Jiang, Hang,Jiang, Mei. 2013

[16]TIP60 contributes to porcine embryonic development by regulating DNA damage response. Guo, Jing,Zhou, Wenjun,Niu, Ying-Jie,Shin, Kyung-Tae,Kim, Nam-Hyung,Cui, Xiang-Shun,Guo, Jing,Heo, Young Tae. 2018

[17]Functional characterization of a cystatin from the tick Rhipicephalus haemaphysaloides. Wang, Yujian,Zhou, Yongzhi,Gong, Haiyan,Cao, Jie,Zhang, Houshuang,Zhou, Jinlin,Wang, Yujian,Li, Xiangrui,Zhou, Jinlin. 2015

[18]Envelope surface ultrastructure and specific gravity of artificially fertilized Pacific cod Gadus macrocephalus eggs. Bian, X.,Jin, X.,Wan, R.,Zhang, X.,Gao, T.,Sakurai, Y.,Yamamoto, J.. 2014

[19]First studies of embryonic and larval development of Coilia nasus (Engraulidae) under controlled conditions. Xu, Gangchun,Tang, Xue,Gu, Ruobo,Xu, Pao,Tang, Xue,Le, Guowei,Zhang, Chengxiang,Zheng, Jinliang. 2011

[20]Effects of low temperatures on sexual reproduction of 'Tainong 1' mango (Mangifera indica). Huang, Jing-Hao,Liang, Guo-Lu,Wang, Wei-Xing,Huang, Jing-Hao,Cai, Zi-Jian,Wen, Shou-Xing,Huang, Jing-Hao,Ma, Wei-Hong,Zhang, Ling-Yuan. 2010

作者其他论文 更多>>

微信小程序