A phosphopantetheinyl transferase that is essential for mitochondrial fatty acid biosynthesis

文献类型： 外文期刊

第一作者： Guan, Xin

作者： Guan, Xin;Nikolau, Basil J.;Guan, Xin;Abramson, Alex;Nikolau, Basil J.;Chen, Hui;Man, Huimin;Wu, Jinxia;Yu, Oliver;Chen, Hui;Man, Huimin;Abramson, Alex;Wu, Jinxia;Yu, Oliver;Nikolau, Basil J.

作者机构：

关键词： phosphopantetheinyl transferase;acyl carrier protein;fatty acid synthase;mitochondria;lipoic acid;glycine decarboxylase;photorespiration;Arabidopsis thaliana

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study we report the molecular genetic characterization of the Arabidopsis mitochondrial phosphopantetheinyl transferase (mtPPT), which catalyzes the phosphopantetheinylation and thus activation of mitochondrial acyl carrier protein (mtACP) of mitochondrial fatty acid synthase (mtFAS). This catalytic capability of the purified mtPPT protein (encoded by AT3G11470) was directly demonstrated in an in vitro assay that phosphopantetheinylated mature Arabidopsis apo-mtACP isoforms. The mitochondrial localization of the AT3G11470-encoded proteins was validated by the ability of their N-terminal 80-residue leader sequence to guide a chimeric GFP protein to this organelle. A T-DNA-tagged null mutant mtppt-1 allele shows an embryo-lethal phenotype, illustrating a crucial role of mtPPT for embryogenesis. Arabidopsis RNAi transgenic lines with reduced mtPPT expression display typical phenotypes associated with a deficiency in the mtFAS system, namely miniaturized plant morphology, slow growth, reduced lipoylation of mitochondrial proteins, and the hyperaccumulation of photorespiratory intermediates, glycine and glycolate. These morphological and metabolic alterations are reversed when these plants are grown in a non-photorespiratory condition (i.e. 1% CO2 atmosphere), demonstrating that they are a consequence of a deficiency in photorespiration due to the reduced lipoylation of the photorespiratory glycine decarboxylase.

分类号： Q94

  • 相关文献

[1]Lipoamide or lipoic acid stimulates mitochondrial biogenesis in 3T3-L1 adipocytes via the endothelial NO synthase-cGMP-protein kinase G signalling pathway. Liu, Jiankang,Shen, Weili,Shen, Weili,Hao, Jiejie,Feng, Zhihui,Tian, Chuan,Feng, Zhihui,Tian, Chuan,Chen, Weijun,Packer, Lester,Shi, Xianglin,Zang, Weijin. 2011

[2]The E-Subgroup Pentatricopeptide Repeat Protein Family in Arabidopsis thaliana and Confirmation of the Responsiveness PPR96 to Abiotic Stresses. Liu, Jia-Ming,Zhao, Juan-Ying,Guo, Chang-Hong,Liu, Jia-Ming,Zhao, Juan-Ying,Lu, Pan-Pan,Chen, Ming,Xu, Zhao-Shi,Ma, You-Zhi. 2016

[3]iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Xu, Wentao,Luo, Yunbo,Huang, Kunlun,Wang, Yan,Wang, Yan,Peng, Xiaoli,Yang, Zhuojun,Zhao, Weiwei,Xu, Wentao,Hao, Junran,Wu, Weihong,Shen, Xiao Li,Luo, Yunbo,Huang, Kunlun,Peng, Xiaoli,Shen, Xiao Li. 2017

[4]Effects of chromium picolinate on fat deposition, activity and genetic expression of lipid metabolism-related enzymes in 21 day old Ross broilers. Chen, Guangxin,Chu, Wenhui,Li, Chunyi,Zhao, Haiping,Chen, Guangxin,Chu, Wenhui,Li, Chunyi,Zhao, Haiping,Chen, Guangxin,Gao, Zhenhua,Cao, Zan. 2018

[5]Manganese influences the expression of fatty acid synthase and malic enzyme in cultured primary chicken hepatocytes. Lu, Lin,Wang, Meiling,Liao, Xiudong,Zhang, Liyang,Luo, Xugang. 2017

[6]The Jatropha curcas KASIII gene alters fatty acid composition of seeds in Arabidopsis thaliana. Yu, N.,Chen, X. Y.,Peng, C. C.,Yu, N.,Xiao, W. F.,Zhu, J.,Chen, X. Y.,Peng, C. C.,Yu, N.,Xiao, W. F.,Zhu, J.,Chen, X. Y.,Peng, C. C.,Xiao, W. F..

[7]Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells. Cai, Xuan,Chen, Xiaolian,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Cai, Xuan,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Chen, Xiaolian. 2013

[8]Photochemical efficiency of PSII and characteristics of photosynthetic CO2 exchange in Indica and Japonica subspecies of rice and their reciprocal cross F-1 hybrids under photoinhibitory conditions. Ji, BH,Jiao, DM. 1999

[9]ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice. Bi, Zhenzhen,Zhang, Yingxin,Wu, Weixun,Zhan, Xiaodeng,Yu, Ning,Xu, Tingting,Liu, Qunen,Li, Zhi,Shen, Xihong,Chen, Daibo,Cheng, Shihua,Cao, Liyong,Bi, Zhenzhen,Zhang, Yingxin,Wu, Weixun,Zhan, Xiaodeng,Yu, Ning,Xu, Tingting,Liu, Qunen,Li, Zhi,Shen, Xihong,Chen, Daibo,Cheng, Shihua,Cao, Liyong.

[10]Contribution of the Alternative Respiratory Pathway to PSII Photoprotection in C3 and C4 Plants. Zhang, Zi-Shan,Liu, Mei-Jun,Zhang, Li-Tao,Yang, Cheng,Meng, Xiang-Long,Gao, Hui-Yuan,Zhang, Zi-Shan,Liu, Mei-Jun,Zhang, Li-Tao,Yang, Cheng,Meng, Xiang-Long,Gao, Hui-Yuan,Zhang, Zi-Shan,Scheibe, Renate,Selinski, Jennifer,Zhang, Li-Tao,Yang, Cheng. 2017

[11]ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice. Zhang, Xiangqian,Ke, Shanwen,Zhu, Haitao,Liu, Fang,Zhang, Zemin,Peng, Xinxiang,Zeng, Ruizhen,Liu, Ziqiang,Zhang, Guiquan,Zheng, Xu,Guo, Lin,Hou, Pei,Wu, Suowei,Song, Meifang,Yang, Jianping,Zhang, Xiangqian.

[12]Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea). Zhou, Ying,Li, Jing,Cheng, Jun,Zhou, Hui,Gu, Chao,Han, Yue-Peng,Guo, Dong,Gardiner, Sue. 2013

[13]Overexpression of the Arabidopsis photorespiratory pathway gene, serine: glyoxylate aminotransferase (AtAGT1), leads to salt stress tolerance in transgenic duckweed (Lemna minor). Yang, Lin,Han, Huajun,Liu, Miaomiao,Zhou, Kaiqiang,Lu, Jianchao,Zhu, Yerong,Bai, Yanling,Wang, Yong,Zuo, Zhaojiang.

[14]The water-water cycle is a major electron sink in Camellia species when CO2 assimilation is restricted. Cai, Yan-Fei,Li, Shu-Fa,Wang, Ji-Hua,Yang, Qiu-Yun,Huang, Wei.

[15]Sulfide-based ATP production in Urechis unicinctus. Ma Zhuojun,Bao Zhenmin,Wang Sifeng,Zhang Zhifeng,Ma Zhuojun,Wang Sifeng. 2010

[16]Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed. Xin, Xia,Tian, Qian,Yin, Guangkun,Chen, Xiaoling,Zhang, Jinmei,Lu, Xinxiong,Tian, Qian,Ng, Sophia,Ng, Sophia. 2014

[17]Mechanism of Free Zn(2+) Enhancing Inhibitory Effects of EGCG on the Growth of PC-3 Cells: Interactions with Mitochondria. Shen, Shengrong,Yu, Haining,Sun, Shili,Das, Undurti N.. 2009

[18]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[19]Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties. Huang, Wenjing,Oo, Thet Lwin,Gu, Minghua,Zhan, Jie,Wang, Aiqin,He, Long-Fei,He, Huyi,He, Long-Fei. 2018

[20]Transport of Calcium Ions into Mitochondria. Xu, Zhaolong,Zhang, Dayong,He, Xiaolan,Huang, Yihong,Shao, Hongbo,Shao, Hongbo. 2016

作者其他论文 更多>>

微信小程序