Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain

文献类型: 外文期刊

第一作者: Jiang, Yi-feng

作者: Jiang, Yi-feng;Xia, Tian-qi;Zhou, Yan-jun;Yu, Ling-xue;Yang, Shen;Huang, Qin-feng;Li, Li-wei;Gao, Fei;Qu, Ze-hui;Tong, Wu;Tong, Guang-zhi;Jiang, Yi-feng;Zhou, Yan-jun;Tong, Guang-zhi

作者机构:

关键词: PRRSV;Genetic diversity;JXA1-P80;Virulence reversion

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Three porcine reproductive and respiratory syndrome viruses (PRRSV), NT1, NT2, and NT3, were isolated from three dying piglets from a single pig farm in Jiangsu Province, China. Whole genome sequencing revealed that the three isolates share the highest homology with JXA1-P80, an attenuated vaccine strain developed by serial passage of highly pathogenic PRRSV JXA1 in MARC-145 cells. More than ten amino acids residues in ORF1a, ORF1b, GP4, and GP5 that were thought to be unique to JXA1 attenuated on MARC-145 cells were each found in the corresponding locations of NT1, NT2, and NT3. In virulence assays, piglets infected with NT1, NT2, or NT3 exhibited clinical signs of disease, including high fever, anorexia, and respiratory distress, leading to the death of the majority of the piglets within two weeks. Collectively, these data indicate that NT1, NT2, and NT3 are highly pathogenic PRRSVs and they are likely to be revertants of the vaccine strain JXA1-P80. (C) 2015 Elsevier B.V. All rights reserved.

分类号: S8

  • 相关文献

[1]Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013. Leng, Chao-liang,Sun, Long,Zhang, Gui-hong,Tong, Guang-zhi,Leng, Chao-liang,Tian, Zhi-jun,Zhang, Wu-chao,Zhang, Hong-liang,Zhai, Hong-yue,An, Tong-qing,Peng, Jin-mei,Ye, Chao,Wang, Qian,Sun, Yan,Li, Lin,Zhao, Hong-yuan,Chang, Dan,Cai, Xue-hui,Tong, Guang-zhi,Tong, Guang-zhi.

[2]Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene. Chen, Jia-zeng,Peng, Jin-mei,Bai, Yun,Wang, Qian,Liu, Yi-min,Zhang, Qiu-yue,Chang, Dan,Zhang, Wu-chao,Zhao, Hong-yuan,Ye, Chao,An, Tong-qing,Cai, Xue-hui,Tian, Zhi-jun,Tong, Guang-zhi.

[3]Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Yan, Li-Ping,Xu, Ao-Tian,Wang, Ya-Xin,Zhang, Shan-Rui,Tong, Guang-Zhi,Tian, Zhi-Jun,Hao, Xiao-Fang,Peng, Jin-Mei,An, Tong-Qing,Wei, Tian-Chao,Cai, Xue-Hui,Feng, Li,Li, Xi,Zhang, Gui-Hong,Zhou, Lun-Jiang. 2009

[4]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[5]Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs. Du, Yijun,Song, Cheng,Yoo, Dongwan,Pei, Yanlong,Hodgins, Douglas C.,Wu, Jiaqiang,Song, Cheng,Welch, Siao-Kun W.,Calvert, Jay G.,Li, Gang. 2009

[6]Identification of immunodominant T-cell epitopes in membrane protein of highly pathogenic porcine reproductive and respiratory syndrome virus. Tong, Guang-zhi. 2011

[7]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[8]Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus. Zhang, Shanrui,Zhou, Yanjun,Jiang, Yifeng,Li, Guoxin,Yan, Liping,Yu, Hai,Tong, Guangzhi. 2011

[9]Interference of porcine reproductive and respiratory syndrome virus replication on MARC-145 cells using DNA-based short interfering RNAs. He, Yun-xia,Hua, Rong-hong,Zhou, Yan-jun,Qiu, Hua-ji,Tong, Guang-zhi. 2007

[10]Mapping codon usage of the translation initiation region in porcine reproductive and respiratory syndrome virus genome. Su, Jun-hong,Li, Ji-dong,Ma, Xu-sheng,Dou, Yong-xi,Luo, Xue-nong,Cai, Xue-peng,Ma, Xiao-xia,He, Ya-li,Li, Ji-dong. 2011

[11]Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped baculovirus vaccine against porcine reproductive and respiratory syndrome virus. Wu, Qunfeng,Xu, Fengqin,Fang, Liurong,Xu, Jinfang,Li, Bin,Jiang, Yunbo,Chen, Huanchun,Xiao, Shaobo,Li, Bin. 2013

[12]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[13]Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design. Xu, Yan-Zhao,Zhou, Yan-Jun,Zhang, Shan-Rui,Jiang, Yi-Feng,Tong, Wu,Yu, Hai,Tong, Guang-Zhi.

[14]Influence of N-linked glycosylation of minor proteins of porcine reproductive and respiratory syndrome virus on infectious virus recovery and receptor interaction. Wei, Zuzhang,Tian, Debin,Sun, Lichang,Lin, Tao,Gao, Fei,Liu, Runxia,Tong, Guangzhi,Yuan, Shishan.

[15]Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine. Dai, Zhihong,Zhang, Zhongqiu,Zhao, Deming,Dai, Zhihong,Wang, Zaishi,Zhang, Quan,Zhang, Zhongqiu,Guo, Pengju. 2011

[16]Production of porcine TNF alpha by ADAM17-mediated cleavage negatively regulates porcine reproductive and respiratory syndrome virus infection. Li, Ren,Guo, Longjun,Gu, Weihong,Luo, Xiaolei,Zhang, Jian,Xu, Yunfei,Tian, Zhijun,Feng, Li,Wang, Yue.

[17]Cloning and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus of the ZJ Strain. Hao, Baocheng,Niu, Dengyuan,Xing, Xiaoyong,Xiang, Haitao,Wen, Fengqin,Fu, Xiaoping,Liang, Jianping,Hu, Yonghao,Niu, Dengyuan,Liang, Jianping. 2014

[18]Production of antisera against porcine haptoglobin: Potential for distinguishing haptoglobin subunits. Yang, Yongqian,An, Tongqing,Liu, Fei,Yuan, Zhonghua,Peng, Jinmei,Wu, Yuquan,Meng, Zhenxiang,Tian, Zhijun,Yang, Yongqian,Wu, Jiang,Zhang, Deli.

[19]Nonstructural Protein 4 of Porcine Reproductive and Respiratory Syndrome Virus Modulates Cell Surface Swine Leukocyte Antigen Class I Expression by Downregulating beta 2-Microglobulin Transcription. Qi, Pengfei,Liu, Ke,Wei, Jianchao,Li, Yuming,Li, Beibei,Shao, Donghua,Wu, Zhuanchang,Shi, Yuanyuan,Tong, Guangzhi,Qiu, Yafeng,Ma, Zhiyong.

[20]Open reading frames 1a and 1b of the porcine reproductive and respiratory syndrome virus (PRRSV) collaboratively initiate viral minus-strand RNA synthesis. Tang, Yan-Dong,Fang, Qiong-Qiong,Liu, Ji-Ting,Wang, Tong-Yun,Wang, Yu,Tao, Ye,Liu, Yong-gang,Cai, Xue-Hui,Liu, Ji-Ting.

作者其他论文 更多>>