Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain
文献类型: 外文期刊
第一作者: Jiang, Yi-feng
作者: Jiang, Yi-feng;Xia, Tian-qi;Zhou, Yan-jun;Yu, Ling-xue;Yang, Shen;Huang, Qin-feng;Li, Li-wei;Gao, Fei;Qu, Ze-hui;Tong, Wu;Tong, Guang-zhi;Jiang, Yi-feng;Zhou, Yan-jun;Tong, Guang-zhi
作者机构:
关键词: PRRSV;Genetic diversity;JXA1-P80;Virulence reversion
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Three porcine reproductive and respiratory syndrome viruses (PRRSV), NT1, NT2, and NT3, were isolated from three dying piglets from a single pig farm in Jiangsu Province, China. Whole genome sequencing revealed that the three isolates share the highest homology with JXA1-P80, an attenuated vaccine strain developed by serial passage of highly pathogenic PRRSV JXA1 in MARC-145 cells. More than ten amino acids residues in ORF1a, ORF1b, GP4, and GP5 that were thought to be unique to JXA1 attenuated on MARC-145 cells were each found in the corresponding locations of NT1, NT2, and NT3. In virulence assays, piglets infected with NT1, NT2, or NT3 exhibited clinical signs of disease, including high fever, anorexia, and respiratory distress, leading to the death of the majority of the piglets within two weeks. Collectively, these data indicate that NT1, NT2, and NT3 are highly pathogenic PRRSVs and they are likely to be revertants of the vaccine strain JXA1-P80. (C) 2015 Elsevier B.V. All rights reserved.
分类号: S8
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