Ectopic Expression of a Proteinase Inhibitor I4 (MtPiI4) Gene from Medicago truncatula Confers Plant Resistance to Pseudomonas syringae pv. Tomato DC3000
文献类型: 外文期刊
第一作者: Sun, Di
作者: Sun, Di;Zhu, Can Can;Wang, Lei;Yang, Lu;Yang, Zhi Min;Chen, Jian;Hu, Liang Bin;Zhou, Zhao Sheng
作者机构:
关键词: Medicago truncatula;MtPiI4;Methyl jasmonate;Proteinase inhibitor;Pseudomonas syringae pv. tomato DC3000
期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Proteinase inhibitors (PIs) play an important role in plant responses to biotic and environmental stimuli, but little is known about the role of PIs in mediating plant immune responses to microbial infection. In this study, a gene named proteinase inhibitor I4 (MtPiI4) was isolated from Medicago truncatula and characterized as a serpin family gene with a typically conserved DUF716 domain. MtPiI4 was differentially expressed in seed, root, leaf, stem and flower tissues. Expression of MtPiI4 was induced by inoculation with a typical bacterial pathogen Pseudomonas syringae pv. tomato DC3000 strain (Pst DC3000). It was also up-regulated by methyl jasmonate (MeJA) treatment. To identify its function in regulating plant immunity against Pst DC3000, we constructed transgenic Arabidopsis plants over-expressing MtPiI4. Compared to wild type, 35S::MtPiI4 plants showed enhanced resistance to Pst DC3000. Expression of JA biosynthetic and responsive genes such as LOX2, PDF1.2, and VSP1 was depressed in 35S::MtPiI4 plants as compared to wild type, suggesting that the JA signaling response was attenuated in 35S::MtPiI4 plants upon Pst DC3000 exposure. Furthermore, over-expression of MtPiI4 led to up-regulation of NPR1 (nonexpressor of pathogenesis-related gene 1-a negative regulator of JA signaling) and down-regulation of MAPK4 (mitogen-activated protein kinase4-a positive regulator of JA signaling). These results indicate that MtPiI4 regulation of plant resistance to Pst DC3000 is involved in the JA signaling transduction pathway.
分类号: Q94`Q7
- 相关文献
作者其他论文 更多>>
-
Metabolomics and ionomics reveal the quality differences among peach, acacia and karaya gums
作者:Zhang, Kaiwei;Yu, Xiangyang;Zhang, Kaiwei;Chen, Meng;Zhang, Xue;Chen, Jian;Chen, Xiaolong;Li, Yong;Yu, Xiangyang;Zhang, Kaiwei;Chen, Meng;Zhang, Xue;Chen, Jian;Chen, Xiaolong;Li, Yong;Yu, Xiangyang;Liu, Xin
关键词:Gum; Metabolomics; Flavonoids; Total phenols content; Metabolites
-
Heterologous Expression of a Potential 'Paulownia fortunei' MYB Factor Gene, PfMYB90, Improves Salt and Cold Tolerance in Arabidopsis
作者:Wang, Hongling;Shi, Shizheng;Huang, Ruifang;Sui, Dezong;Gao, Yunpeng;Wang, Lei;Luo, Guijie
关键词:
Paulownia fortunei ;PfMYB90 ; salt stress; cold stress -
Fish-derived cationic antimicrobial peptide piscidin 1 exerts broad-spectrum bactericidal activities against multidrug-resistant Gram-negative pathogens
作者:Sun, Huarun;Zhang, Jingao;Li, Xiaolu;Gao, Haolei;Liao, Chengshui;Wang, Ruibiao;Wen, Bo;Luo, Weiyu;Zhang, Wei;Zhu, Chunling;Wen, Yuliang;Zhang, Longfei;Liu, Xuehan;Shen, Jiyuan;Hu, Jianhe;Bai, Yueyu;Zhang, Huihui;Ding, Ke;Wang, Lei;Sun, Huarun;Zhang, Jingao;Gao, Haolei;Wang, Ruibiao;Wen, Bo;Luo, Weiyu;Zhang, Wei;Zhu, Chunling;Wen, Yuliang;Zhang, Longfei;Liu, Xuehan;Shen, Jiyuan;Hu, Jianhe;Zhang, Huihui;Wang, Lei;Liao, Chengshui;Ding, Ke;Wu, Yundi;Wu, Xilong;Li, Hao
关键词:Antimicrobial peptide; Multiresistance; Piscidin 1; Bactericidal mechanism; Gram-negative bacteria
-
Soybean yield estimation and lodging classification based on UAV multi-source data and self-supervised contrastive learning
作者:Zhou, Longyu;Chen, Haochong;Feng, Puyu;Ma, Yuntao;Zhang, Yong;Wang, Lei;Li, Mingxue;Sun, Xuhong;Sun, Guangyao;Yan, Long;Qiu, Lijuan;Li, Yinghui
关键词:Remote sensing; 3D reconstruction; Point cloud; Multispectral; Structure from motion
-
Effects of Artemisia argyi leaf water extract (AWE) on growth performance muscle quality, intestinal microbial, and metabolomics of common carp ( Cyprinus carpio)
作者:Sun, Di;Zeng, Nanyang;Pan, Yingjia;Shu, Qingsong;Zou, Jixing;Shi, Jun;Huang, Yingshi;Wang, Guoxia;Shi, Jun
关键词:Plant extracts; Flesh quality; Antioxidant capacity; Growth performance
-
A Comparative Analysis of Different Algorithms for Estimating Evapotranspiration with Limited Observation Variables: A Case Study in Beijing, China
作者:Sun, Di;Zhang, Hang;Qi, Yanbing;Li, Xuemin;Qi, Yanbing;Ren, Yanmin;Zhang, Zhengxian;Lv, Yuping;Cheng, Minghan;Cheng, Minghan
关键词:evapotranspiration; LST-VI space; long-time-series; machine learning; spatial distribution
-
Bridging the gap between hyperspectral imaging and crop breeding: soybean yield prediction and lodging classification with prototype contrastive learning
作者:Sun, Guangyao;Han, Shiteng;Xu, Yun;Ma, Yuntao;Zhang, Yong;Wang, Lei;Zhou, Longyu;Fei, Shuaipeng;Xiao, Shunfu;Ma, Yuntao;Yan, Long;Che, Yingpu;Li, Yinghui;Qiu, Lijuan
关键词:Unmanned Aerial Vehicle; Deep Learning; Resnet; Soybean Yield and Lodging Prediction