Isolation and Functional Analysis of Convolvulus arvensis EPSPS Promoter

文献类型： 外文期刊

第一作者： Wang, Guirong

作者： Wang, Guirong;Huang, Hongjuan;Wei, Shouhui;Zhou, Xinxin;Chen, Jinyi;Chen, Jingchao;Zhang, Chaoxian

作者机构：

关键词： Glyphosate;Arabidopsis thaliana;Promoter;Transgenic plant

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

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收录情况： SCI

摘要： A 1142-bp upstream sequence (named CaEPSPS-P, GenBank accession number: KC107822) of the EPSPS gene from Convolvulus arvensis L was obtained by genome walking. The full-length sequence of the CaEPSPS-P and four deletion mutants were fused to the beta-glucuronidase (GUS) gene and introduced into Arabidopsis via Agrobacterium-mediated transformation. Histochemical GUS staining of the transgenic plants showed that the CaEPSPS-P could drive GUS expression in the roots, stems, and leaves, but no visible GUS staining was detected in seeds. Further deletion analysis revealed two positive regulatory regions (-900 to -632 and -632 to -418) responsible for the basal activity of the EPSPS promoter. GUS activity assays indicated that GUS expression can be stimulated by light and glyphosate. In addition, a region between -632 and -400 was necessary for light-induced expression, while a region from -900 to -632 was necessary for glyphosate-induced GUS expression. These results suggested that the CaEPSPS-P was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate transgene expression.

分类号： Q94`Q7

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