文献类型: 外文期刊
第一作者: Hu, Xianqiao
作者: Hu, Xianqiao;Lu, Lin;Fang, Changyun;Duan, Binwu;Zhu, Zhiwei;Hu, Xianqiao;Lu, Lin;Fang, Changyun;Duan, Binwu;Zhu, Zhiwei
作者机构:
关键词: apparent amylose content;paper-based microfluidic chips;rice;color reaction
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Determination of apparent amylose content in rice is a key function for rice research and the rice industry. In this paper, a novel approach with paper-based microfluidic chip is reported to determine apparent amylose content in rice. The conventional color reaction between amylose and iodine was employed. Blue color of amylose iodine complex generated on-chip was converted to gray and measured with Photoshop after the colored chip was scanned. The method for preparation of the paper chip is described. In situ generation of iodine for on-chip color reaction was designed, and factors influencing color reaction were investigated in detail. Elimination of yellow color interference of excess iodine by exploiting color removal function of Photoshop was presented. Under the optimized conditions, apparent amylose content in rice ranging from 1.5 to 26.4% can be determined, and precision was 6.3%. The analytical results obtained with the developed approach were in good agreement with those with the continuous flow analyzer method.
分类号: R15`S
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