Establishment and evaluation of a murine alpha v beta 3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus

文献类型： 外文期刊

第一作者： Zhang, Wei

作者： Zhang, Wei;Lian, Kaiqi;Yang, Fan;Yang, Yang;Zhu, Zhijian;Zhu, Zixiang;Cao, Weijun;Mao, Ruoqing;Jin, Ye;He, Jijun;Guo, Jianhong;Liu, Xiangtao;Zheng, Haixue

作者机构：

关键词： alpha v beta 3;cell line;Foot-and-mouth disease virus;integrin;receptor

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Integrin alpha v beta 3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of alpha v beta 3 integrin, a stable CHO-677 cell line expressing the murine alpha v beta 3 heterodimer (designated as "CHO-677-m alpha v beta 3" cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits alpha v and beta 3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-m alpha v beta 3 cell line at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed stably. Aplaque-forming assay, 50% tissue culture infective dose (TCID50), real-time quantitative reverse transcription-PCR, and IFA were used to detect the replication levels of Foot-and-mouth disease virus (FMDV) in the CHO-677-m alpha v beta 3 cell line. After infection with FMDV/O/ZK/93, the cell line showed a significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stable alpha v beta 3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of alpha v beta 3 integrin, and as a cell model for FMDV research.

分类号： S8

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