A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp (Cyprinus carpio var. Jian) Using Precision-Cut Liver Slices

文献类型： 外文期刊

第一作者： Du, Jin-Liang

作者： Du, Jin-Liang;Cao, Li-Ping;Yin, Guo-Jun;Du, Jin-Liang;Cao, Li-Ping;Yin, Guo-Jun;Liu, Ying-Juan;Jia, Rui;Yin, Guo-Jun

作者机构：

关键词： Jian Carp;2;3;7;8-Tetrachlorodibenzo-p-dioxin;AhR2;ARNT2;CYP1A

期刊名称：BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY （ 影响因子：2.151； 五年影响因子：2.129 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to establish a model for the study of liver injury induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Jian carp using precision-cut liver slices (PCLS). PCLS were treated with TCDD at concentrations of 0, 0.05, 0.1, 0.3, and 0.6 mu g/L for 6 h, followed by collection of the culture supernatant and PCLS for analysis. Several biochemical indices were analyzed, including glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). Expression of mRNA was also estimated for cytochrome P4501A (CYP1A), aryl hydrocarbon receptor2 (AhR2), and aryl hydrocarbon receptor nuclear translocator2 (ARNT2). Results showed that some significant effects (p < 0.05) in MDA, GSH-Px and PCLS viability were observed at a TCDD concentration as low as 0.05 mu g/L, and the observed effects increased with exposure concentration. Following exposure to TCDD for 6 h at a concentration of 0.3 mu g/L, significant increases (p < 0.01) in the content of GPT, GOT, MDA, and LDH were observed, while SOD activity, GSH-Px activity, and PCLS viability were decreased (p < 0.01 or p < 0.05). Exposure to 0.3 mu g/L TCDD also resulted in increased expression of mRNA for CYP1A, AhR2, and ARNT2. Overall, these results provide evidence of TCDD-induced liver injury and oxidative stress in Jian carp. These results also support the use of PCLS as an in vitro model for the evaluation of hepatotoxicity in Jian carp.

分类号： R99

  • 相关文献

[1]Design, synthesis and fungicidal activity of N-substituted benzoyl-1,2,3,4-tetrahydroquinolyl-1-carboxamide. Xu, Yan,Du, Juan,Yang, Xin-Ling,Xu, Gao-Fei,Ling, Yun,Yuan, Hui-Zhu.

[2]Superoxide radical and auxin are implicated in redistribution of root growth and the expression of auxin and cell-cycle genes in cadmium-stressed rice. Zhao, F. Y.,Hu, F.,Han, M. M.,Liu, W.,Zhang, S. Y..

[3]Synthesis and antibacterial activity of oxime ester derivatives containing 1,2,4-triazole or 1,3,4-oxadiazole moiety. Wang, Xiaobin,Zhong, Xinmin,Zhu, Xuesong,Li, Qin,Zhang, Juping,Ruan, Xianghui,Xue, Wei,Wang, Hua.

[4]Rhizosphere isoflavones (daidzein and genistein) levels and their relation to the microbial community structure of mono-cropped soybean soil in field and controlled conditions. Guo, Zhong-Yuan,Kong, Chui-Hua,Wang, Jing-Guo,Wang, Yu-Feng.

[5]Identification of Tartary Buckwheat Tea Aroma Compounds with Gas Chromatography-Mass Spectrometry. Qin, Peiyou,Wu, Li,Ren, Guixing,Ma, Tingjun,Shan, Fang.

[6]Over-expression of (1,3;1,4)--D-glucanase isoenzyme EII gene results in decreased (1,3;1,4)--D-glucan content and increased starch level in barley grains. Han, Ning,Na, Chenglong,Chai, Yuqiong,Chen, Jianshu,Zhang, Zhongbo,Bai, Bin,Bian, Hongwu,Zhu, Muyuan,Zhang, Yuhong.

[7]Transport via xylem of atrazine, 2,4-dinitrotoluene, and 1,2,3-trichlorobenzene in tomato and wheat seedlings. Su, Yu H.,Liang, Yong C.,Liang, Yong C..

[8]Expression and characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas. Xiong, Fei,Shuai, Jian-Jun,Sun, Jing,Xiong, Ai-Sheng,Shuai, Jian-Jun,Jin, Xiao-Fen,Sun, Jing,Peng, Ri-He,Yao, Quan-Hong,Zhang, Jian.

[9]A novel thermal iniferter for radical polymerization. Chen, XP,Qiu, KY,Swift, G,Westmoreland, DG,Wu, SQ.

[10]Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var. Jian) compared with mirror carp (Cyprinus carpio L.). Gu Ying,Lu Cuiyun,Zhang Xiaofeng,Li Chao,Sun Xiaowen,Lu Cuiyun,Yu Juhua. 2015

[11]Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity. Xu, Hongyan,Li, Caixia,Li, Yan,Ng, Grace Hwee Boon,Liu, Chunsheng,Zhang, Xiaoyan,Gong, Zhiyuan,Xu, Hongyan,Liu, Chunsheng.

[12]Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. Lim, Yourim,Park, Ji-Won,Kwon, Ok-Kyoung,Lee, Jae-Won,Lee, Han-Sol,Ahn, Kyung-Seop,Lim, Yourim,Lee, Han-Sol,Han, Sang-Bae,Lee, Sangwoo,Choi, Sangho,Li, Wanyi,Jin, Hang. 2018

[13]Cellular and ultrastructural changes in the seedling roots of upland rice (Oryza sativa) under the stress of two allelochemicals from Ageratina adenophora. Wan, Fang-Hao,Guo, Jian-Ying,Liu, Wan-Xue,Yang, Guo-Qing.

[14]One new flavonoid from Solanum rostratum. Wang, Hui-min,Cao, Ao-cheng,Zhang, Chao-xian,Wei, Shou-hui,Ling, Tie-jun. 2017

[15]5,6,7,8-Tetrahydro-2-(2-phenylethyl)chromones from artificial agarwood of Aquilaria sinensis and their inhibitory activity against acetylcholinesterase. Mei, Wen-Li,Kong, Fan-Dong,Li, Wei,Yuan, Jing-Zhe,Dai, Hao-Fu,Liao, Ge.

[16]Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Xu, Gangming,Li, Yingying,Zheng, Wei,Peng, Xiang,Li, Wen,Yan, Yanchun.

[17]Structural diversity-guided convenient construction of functionalized 4H-1,3,4-oxadiazin-5(6H)-one derivatives. Ke, Shaoyong,Wang, Kaimei,Zhang, Zhigang,Zhang, Ya-Ni,Liang, Ying,Shi, Liqiao,Jiang, Aibing,Yang, Ziwen.

[18]Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest. Wu, Kongming,Wang, Guirong,Li, Fengqi,Li, Wei,Lin, Yong-Jun,Pickett, John A.,Birkett, Michael A.,Zhou, Jing-Jiang. 2018

[19]Design, Synthesis and Bioactivity of Novel Glycosylthiadiazole Derivatives. Zong, Guanghui,Zhao, Hanqing,Jiang, Rui,Zhang, Jianjun,Liang, Xiaomei,Wang, Daoquan,Zhao, Hanqing,Li, Baoju,Shi, Yanxia.

[20]Influences of nitrification inhibitor 3,4-dimethyl pyrazole phosphate on nitrogen and soil salt-ion leaching. Yu Qiaogang,Ye Xuezhu,Yu Qiaogang,Chen Yingxu,Zhang Zhijian,Tian Guangming.

作者其他论文 更多>>

微信小程序