Biochemical characterization of an acidophilic beta-mannanase from Gloeophyllum trabeum CBS900.73 with significant transglycosylation activity and feed digesting ability

文献类型： 外文期刊

第一作者： Wang, Caihong

作者： Wang, Caihong;Wang, Yuan;Niu, Canfang;Ma, Rui;Wang, Yaru;Bai, Yingguo;Luo, Huiying;Yao, Bin;Zhang, Jiankang;Ma, Rui

作者机构：

关键词： Gloeophyllum trabeum;beta-Mannanase;Acidophilic;Broad pH adaptability/stability;Transglycosylation

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Acidophilic beta-mannanases have been attracting much attention due to their excellent activity under extreme acidic conditions and significant industrial applications. In this study, a beta-mannanase gene of glycoside hydrolase family 5, man5A, was cloned from Gloeophyllum trabeum CBS900.73, and successfully expressed in Pichia pastoris. Purified recombinant Man5A was acidophilic with a pH optimum of 2.5 and exhibited great pH adaptability and stability (>80% activity over pH 2.0-6.0 and pH 2.0-10.0, respectively). It had a high specific activity (1356 U/mg) against locust bean gum, was able to degrade galactomannan and glucomannan in a classical four-site binding mode, and catalyzed the transglycosylation of mannotetrose to mannooligosaccharides with higher degree of polymerization. Besides, it had great resistance to pepsin and trypsin and digested corn-soybean meal based diet in a comparable way with a commercial beta-mannanase under the simulated gastrointestinal conditions of pigs. This acidophilic beta-mannanase represents a valuable candidate for wide use in various industries, especially in the feed. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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