Optimization of selenylation modification for garlic polysaccharide based on immune-enhancing activity
文献类型: 外文期刊
第一作者: Gao, Zhenzhen
作者: Gao, Zhenzhen;Qiu, Shulei;Li, Youying;Wang, Deyun;Liu, Cui;Li, Xiuping;Hou, Ranran;Yue, Chanjuan;Liu, Jie;Hu, Yuanliang;Chen, Jin;Li, Hongquan
作者机构:
关键词: Garlic polysaccharide;Selenylation modification;Lymphocyte proliferation;Cytokines Immune response
期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Garlic polysaccharide (GPS) was modified in selenylation respectively by nitric acid-sodium selenite (NA-SS), glacial acetic acid-selenous acid (GA-SA), glacial acetic acid-sodium selenite (GA-SS) and selenium oxychloride (SOC) methods each under nine modification conditions of L-9 (3(4)) orthogonal design and each to obtain nine selenizing GPSs (sGPSs). Their structures were identified, yields and selenium contents were determined, selenium yields were calculated, and the immune-enhancing activities of four sGPSs with higher selenium yields were compared taking unmodified GPS as control. The results showed that among four methods the selenylation efficiency of NA-SS method were the highest, the activity of sGPS(5) was the strongest and significantly stronger than that of unmodified GPS. This indicates that selenylation modification can significantly enhance the immune-enhancing activity of GPS, NA-SS method is the best method and the optimal conditions are 0.8:1 weight ratio of sodium selenite to GPS, reaction temperature of 70 degrees C and reaction time of 10 h. (C) 2015 Elsevier Ltd. All rights reserved.
分类号: O629`Q53
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