CYP287A1 is a carotenoid 2-beta-hydroxylase required for deinoxanthin biosynthesis in Deinococcus radiodurans R1

文献类型: 外文期刊

第一作者: Zhou, Zhengfu

作者: Zhou, Zhengfu;Zhang, Wei;Su, Shiyou;Chen, Ming;Lu, Wei;Lin, Min;Xu, Yuquan;Molnar, Istvan

作者机构:

关键词: Deinococcus radiodurans;Deinoxanthin;CYP287A1;Cytochrome P450;2-beta-hydroxylase

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

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收录情况: SCI

摘要: The carotenoid deinoxanthin is a crucial resistance factor against various stresses in the radiation-resistant bacterium Deinococcus radiodurans. Disruption of the gene dr2473 encoding the cytochrome P450 CYP287A1 led to the accumulation of 2-deoxydeinoxanthin in D. radiodurans, demonstrating that CYP287A1 is a novel beta-carotene 2-hydroxylase. The dr2473 knockout mutant was shown to be more sensitive to UV radiation and oxidative stress than the wild-type strain D. radiodurans R1, indicating that the C2 alcohol of deinoxanthin is important for antioxidant activity.

分类号: Q939.9`Q81

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