Discovery and functional characterization of leptin and its receptors in Japanese quail (Coturnix japonica)

文献类型： 外文期刊

第一作者： Wang, Dandan

作者： Wang, Dandan;Xu, Chunlin;Wang, Taian;Li, Hong;Li, Yanmin;Ren, Junxiao;Tian, Yadong;Li, Zhuanjian;Jiao, Yuping;Kang, Xiangtao;Liu, Xiaojun;Tian, Yadong;Li, Zhuanjian;Kang, Xiangtao;Liu, Xiaojun;Tian, Yadong;Li, Zhuanjian;Kang, Xiangtao;Liu, Xiaojun;Jiao, Yuping

作者机构：

关键词： Quail;Leptin;LEPR;Gene cloning;Alternative splicing;Physiological function

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Leptin is an important endocrine regulation factor of food intake and energy homeostasis in mammals; however, the existence of a poultry leptin gene (LEP) is still debated. Here, for the first time, we report the cloning of a partial exon 3 sequence of LEP (qLEP) and four different leptin receptor splicing variants, including a long receptor (qLEPRl) and three soluble receptors (qLEPR-a, qLEPR-b and qLEPR-c) in Japanese quail (Coturnix japonica). The qLEP gene had high GC content (64%), which is similar to other reported avian leptin genes. The encoded qLEP protein possessed the conserved pair of cysteine residues that are required to form a lasso knot for full biological activity, but shared relatively low identities with LEPs of other vertebrates. The translated qLEPR1 protein contained 1143 amino acids and shared high amino acid sequence identity with a chicken homolog (89% identity). qLEPR1 also contained all the motifs, domains, and basic tyrosine residues that are conserved in the LEPRI proteins of other vertebrates. qRT-PCR analysis showed that LEP and the four LEPR variants were expressed extensively in all tissues examined; the expression levels of LEP were relatively high in hypothalamus, skeletal muscle, and pancreas, while the expression levels of the LEPRs were highest in the pituitary. Compared with the expression levels of juvenile qLEP and total qLEPR (including all LEPR variants), the expression levels of mature qLEP and total qLEPR were up-regulated in the hypothalamus and pituitary, and down-regulated in the ovary. The expressions of LEP/LEPR increased when fasting and decreased when refeeding in the brain and peripheral tissues of juvenile quail, which suggested that the LEP/LEPR system modulated food intake and energy expenditure, although, unlike in mammals, LEP may actually act to inhibit food intake during fasting, at least in juvenile quail. The results indicate that qLEP and qLEPR have unique expression patterns and that the encoded proteins play important roles in the regulation of reproduction and energy status in Japanese quail. (C) 2015 Elsevier Inc. All rights reserved.

分类号： R58

  • 相关文献

[1]Alternative splicing and tissue expression of CIB4 gene in sheep testis. Yu, Yan,Wei, Caihong,Lu, Guobin,Zhang, Ju,Ren, Hangxing,Sheng, Xihui,Du, Lixin,Yu, Yan,Guan, Qun,Zhang, Qiyao,Zhang, Yuan,Wang, Chuduan,Song, Xuemei,Jin, Mei,Li, Shangang. 2010

[2]Invertebrate Trehalose-6-Phosphate Synthase Gene:Genetic Architecture, Biochemistry, Physiological Function, and Potential Applications. Tang, Bin,Wang, Shi-Gui,Wang, Hui-Juan,Tang, Bin,Cui, Shuai-Ying,Wang, Su,Zhang, Jia-Yong. 2018

[3]Dietary L-alanyl-L-glutamine supplementation improves growth performance and physiological function of hybrid sturgeon Acipenser schrenckii female x A. baerii male. Wang, C. -A.,Xu, Q. -Y.,Xu, H.,Zhu, Q.,Yang, J. -L.,Sun, D. -J..

[4]Effect of chicken leptin recptor short hairpin RNA on expression of JAK2, STAT3, SOCS3 and CPT1 genes in chicken preadipocytes. Ban, Qian,Cheng, Fei,Liu, Dahai,Hui, Wenqiao,Liu, Xiaojun,Ban, Qian,Hui, Wenqiao,Liu, Xiaojun.

[5]Long form leptin receptor and SNP effect on reproductive traits during embryo attachment in Suzhong sows. Fu, Yanfeng,Li, Bixia,Fang, Xiaomin,Ren, Shouwen,Fu, Yanfeng,Li, Lan,Li, Lan.

[6]The quest of influenza A viruses for new hosts. Liu, M,Guan, Y,Peiris, M,He, S,Webby, RJ,Perez, D,Webster, RG. 2003

[7]Protective Efficacy of an H5N1 DNA Vaccine Against Challenge with a Lethal H5N1 Virus in Quail. Li, Junping.

[8]Discovery and characterization of Coturnix chinensis avian ss-defensin 10, with broad antibacterial activity. Ma, Deying,Lin, Lijuan,Zhang, Kexin,Wang, Ruiqin,Lin, Lijuan,Zhang, Kexin,Han, Zongxi,Shao, Yuhao,Wang, Ruiqin,Liu, Shengwang. 2012

[9]The influenza virus gene pool in a poultry market in South Central China. Liu, M,He, SQ,Walker, D,Zhou, NN,Perez, DR,Mo, B,Li, F,Huang, XT,Webster, RG,Webby, RJ. 2003

[10]Effects of dietary taurine on egg production, egg quality and cholesterol levels in Japanese quail. Wang, Fu-Rong,Zhang, Xiao-Ming,Tong, Jian-Ming,Xie, Zhong-Guo,Wang, Fu-Rong,Zhang, Xiao-Ming,Tong, Jian-Ming,Xie, Zhong-Guo,Wang, Fu-Rong,Dong, Xiao-Fang,Tong, Jian-Ming,Zhang, Qi. 2010

[11]Effects of dietary taurine supplementation on growth performance and immune status in growing Japanese quail (Coturnix coturnix japonica). Wang, F. R.,Tong, J. M.,Zhang, X. M.,Wang, F. R.,Tong, J. M.,Zhang, X. M.,Wang, F. R.,Dong, X. F.,Tong, J. M.,Zhang, Q.,Wu, Y. Y.. 2009

[12]Identification and characterization of an avian beta-defensin orthologue, avian beta-defensin 9, from quails. Wang, Ruiqin,Ma, Deying,Lin, Lijuan,Zhou, Caiyuan,Liao, Wenyan,Han, Zongxi,Shao, Yuhao,Liu, Shengwang,Liu, Shengwang. 2010

[13]Purple sweet potato (Ipomoea batatas L.) color alleviates high-fat-diet-induced obesity in SD rat by mediating leptin's effect and attenuating oxidative stress. Zhang, Yi,Niu, FuXiang,Sun, Jian,Xu, Fei,Yue, RuiXue. 2015

[14]Effects of feeding corn naturally contaminated with aflatoxin on growth performance, apparent ileal digestibility, serum hormones levels and gene expression of Na+, K+-ATPase in ducklings. Abbasi, Farzana,Liu, Jingbo,Shen, Xiaoyun,Luo, Xuegang,Liu, Jingbo,Zhang, Hongfu,Shen, Xiaoyun. 2018

[15]Association of the leptin gene E2-169T > C and E3-299T > A mutations with carcass and meat quality traits of the Chinese Simmental-cross steers. Tian, Jing,Zhao, Zhihui,Yu, Zhongjiang,Yang, Runjun,Zhang, Lupei,Li, Junya,Zhang, Qingfeng. 2013

[16]Dietary arginine from 15 to 35 days of age affects feed intake and carcass yield in White Pekin ducks. Wang, Chao,Xie, Ming,Huang, Wei,Yu, Jun-Ying,Feng, Yu-Long,Hou, Shui-Sheng,Wang, Chao. 2013

[17]Milk progesterone on day 5 following insemination in the dairy cow: associated metabolic variables and reproductive consequences. Yan, L. Y.,Shi, Z. D.,Robinson, R. S.,Mann, G. E.. 2018

[18]Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation. Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Zang, Kun,Ling, Zongshuai. 2012

[19]Molecular cloning, characterization and expression analysis of multiple leptin genes in Jian carp (Cyprinus carpio var. Jian). Tang, Yongkai,Yu, Juhua,Xu, Pao,Tang, Yongkai,Yu, Juhua,Li, Hongxia,Xu, Pao,Li, Jianlin,Ren, Hongtao. 2013

[20]Effect of Additional Boron on Tibias of African Ostrich Chicks. Cheng, Jiayue,Peng, Kemei,Song, Hui,Liu, Huazhen,Cheng, Jiayue,Tang, Zhonglin,Jin, Erhui,Zhang, Yong,Liu, Yan,Zhang, Ningbo. 2011

作者其他论文 更多>>

微信小程序