Designed diblock hairpin probes for the nonenzymatic and label-free detection of nucleic acid

文献类型: 外文期刊

第一作者: Wen, Junlin

作者: Wen, Junlin;Wen, Junlin;Chen, Junhua;Zhuang, Li;Zhou, Shungui;Wen, Junlin

作者机构:

关键词: Colorimetric assay;Branched DNA junction;Gold nanoparticles;Catalytic hairpin assembly;Shewanella oneidensis

期刊名称:BIOSENSORS & BIOELECTRONICS ( 影响因子:10.618; 五年影响因子:9.323 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The detection of nucleic acid sequences is of great importance in a variety of fields. An ultrasensitive DNA sensing platform is constructed using elaborately designed diblock hairpin probes (DHPs) that are composed of hairpin and poly-adenine blocks. The introduction of an initiator DNA target triggers the catalytic assembly of probes DHP1, DHP2 and DHP3 to fabricate numerous poly-adenine-tailed branched DNA junctions, which significantly amplify the signal of the target-DNA-recognizing event without any enzyme. Coupled to a gold nanoparticle-based colorimetric assay, the amplified recognition signal can be quantitatively detected or visually read with the naked eye. The combination of the high-efficiency target-catalyzed DHP assembly and sensitive gold-based colorimetric assay offers an ultrasensitive detection of DNA with a detection limit of 0.1 pM and a dynamic range from 0.01 to 5 pM. The proposed sensing platform can discriminate even single-base mutations. Moreover, the sensing platform can be expanded to detect pollutant-degrading-bacteria-specific DNA sequences. The proposed sensing system should offer an alternative approach for the detection of nucleic acids in the fields of microbiology, biogeochemistry, and environmental sciences. (C) 2016 Published by Elsevier B.V.

分类号: Q

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