Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX

文献类型： 外文期刊

第一作者： Che Jianmei

作者： Che Jianmei;Liu Bo;Chen Zheng;Shi Huai;Liu Guohong;Ge Cibin

作者机构：

关键词： Ethylparaben;Brevibacillus brevis;Antimicrobial activity;Purification

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, crude antimicrobial extract from the culture supernatant of Brevibacillus brevis FJAT-0809-GLX was extracted, and its antimicrobial activity was investigated with the agar diffusion method. The results showed that the antimicrobial activity of the culture supernatant of B. brevis FJAT-0809-GLX increased with the extension of the incubation time of B. brevis FJAT-0809-GLX. The antimicrobial spectrum assays showed that this crude antimicrobial extract from culture supernatant of B. brevis FJAT-0809-GLX could inhibit the growth of both bacteria and fungi. A heat stability test was performed, and different temperatures (30 degrees C, 50 degrees C and 70 degrees C) did not affect the antibiotic activity of this crude antimicrobial extract. The crude antimicrobial extract was also tolerable to changes in pH levels. Its antibiotic activity against Escherichia coli was stable at pH 1 to pH 11, with zone sizes ranging from 18.46 mm to 22.19 mm. Almost all of the crude extracts extracted using different solvents showed variable degrees of inhibition zones against E. coli, with zone sizes ranging from 17.29 mm to 19.62 mm, except petroleum ether and butanol extracts, which were found to be completely inactive. Purification of the antimicrobial components was carried out using a column chromatographic technique with column chromatography grade silica gel and analyzed by an Agilent 7890A Network GC system. The separated compound was identified as ethylparaben, with a retention time of 21.980 min and a relative amount of 95.50%. The antimicrobial activity of ethylparaben on different types of bacteria and fungi was investigated, and ethylparaben was shown to inhibit different types of microbes to different extents. To the best of our knowledge, this is the first report demonstrating that the bacterium B. brevis could produce ethylparaben. (C) 2014 Elsevier GmbH. All rights reserved.

分类号： Q

  • 相关文献

[1]Tostadin, a novel antibacterial peptide from an antagonistic microorganism Brevibacillus brevis XDH. Liu, Xunli,Song, Zhen,Guo, Hui,Ju, Ruicheng,Zhao, Yuhua,Guo, Hui.

[2]Purification and characterization of a novel antimicrobial peptide from Brevibacillus laterosporus strain A60. Guo, Lihua,Zeng, Hongmei,Yang, Xiufen,Yuan, Jingjing,Shi, Huaixing,Xiong, Yehui,Chen, Mingjia,Han, Lei,Qiu, Dewen.

[3]Effects of Brevibacillus brevis FJAT-1501-BPA on growth performance, faecal microflora, faecal enzyme activities and blood parameters of weaned piglets. Che, Jianmei,Liu, Bo,Deng, Yuanyuan,Chen, Qianqian,Ge, Cibin,Liu, Guohong,Wang, Jieping,Ye, Shaowen.

[4]Biocontrol of Lasiodiplodia theobromae, which causes black spot disease of harvested wax apple fruit, using a strain of Brevibacillus brevis FJAT-0809-GLX. Che, Jianmei,Liu, Bo,Ruan, Chuanqing,Tang, Jianyang,Huang, Dandan.

[5]Molecular analysis and recombinant expression of bovine neutrophil beta-defensin 12 and its antimicrobial activity. Wu, Jianming,Wang, Changfa,He, Hongbin,Yang, Hongjun,Gao, Yundong,Zhong, Jifeng,Hu, Guixue. 2011

[6]Staurosporine from the endophytic Streptomyces sp strain CNS-42 acts as a potential biocontrol agent and growth elicitor in cucumber. Xiao, Lie,Zhang, Bo,Zhang, Xiaoping,Li, Xiaolin,Zheng, Linyong,Gan, Bingcheng,Huang, Pei,Wang, Qian,Liu, Miaomiao,Bolla, Krishna,Liu, Xueting,Zhang, Lixin,Huang, Pei,Wang, Qian,Liu, Xueting. 2014

[7]Structure-function analysis of Avian beta-defensin-6 and beta-defensin-12: role of charge and disulfide bridges. Yang, Ming,Zhang, Chunye,Zhang, Shuping,Zhang, Xuehan,Zhang, Michael Z.,Rottinghaus, George E.,Zhang, Michael Z.,Rottinghaus, George E.,Zhang, Shuping. 2016

[8]The pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp lycopersici. Xu, Jianhong,Xu, Jianhong,Deng, Peng,Baird, Sonya M.,Lu, Shi-En,Showmaker, Kurt C.,Wang, Hui. 2014

[9]In vitro Antimicrobial Activities and Mechanism of 1-Octen-3-ol against Food-related Bacteria and Pathogenic Fungi. Li, Qiang,Chen, Zuqin,Xiong, Chuan,Li, Qiang,Huang, Wenli,Li, Shuhong,Chen, Cheng. 2017

[10]A new fungicide produced by a Streptomyces sp GAAS7310. Chen, GY,Lin, BR,Lin, YC,Xie, FC,Lu, W,Fong, WF. 2005

[11]New Azalomycin F Analogs from Mangrove Streptomyces sp 211726 with Activity against Microbes and Cancer Cells. Yuan, Ganjun,Yuan, Ganjun,Hong, Kui,Lin, Haipeng,Yuan, Ganjun,Hong, Kui,She, Zhigang,Li, Jia. 2013

[12]Comparison of Compositions and Antimicrobial Activities of Essential Oils from Chemically Stimulated Agarwood, Wild Agarwood and Healthy Aquilaria sinensis (Lour.) Gilg Trees. Chen, Huaiqiong,Yang, Yun,Xue, Jian,Wei, Jianhe,Zhang, Zheng,Chen, Hongjiang,Chen, Huaiqiong,Yang, Yun,Xue, Jian,Wei, Jianhe,Zhang, Zheng,Chen, Hongjiang,Yang, Yun,Chen, Hongjiang,Yang, Yun,Chen, Hongjiang.

[13]A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action. Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Wang, Qing,Wu, Huifeng,Pei, Dong,Cong, Ming,Li, Fei,Ji, Chenglong,Zhao, Jianmin,Zhang, Linbao,Yang, Dinglong,Yuan, Zeyi.

[14]Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities. Xiang, Jinsong,Li, Xihong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Zhang, Wenting,Zeng, Yan,Sun, Luming,Chen, Song Lin,Sha, Zhenxia,Xiang, Jinsong,Chen, Xuejie,Xiang, Jinsong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Sha, Zhenxia,Li, Xihong,Chen, Song Lin,Yu, Mengjun,Zhang, Wenting,Zeng, Yan,Sun, Luming.

[15]Antibacterial Activity and Action Mechanism of the Echinops ritro L. Essential Oil Against Foodborne Pathogenic Bacteria. Jiang, Bin,Wang, Fei,Liu, Lei,Tian, Shangyi,Li, Wenliang,Huang, Yanxin,Yi, Jingwen,Li, Yuxin,Li, Wenliang,Wu, Yin,Yu, Chunlei,Sun, Luguo,Yang, Xiaoguang,Zhang, Yuwei.

[16]Contribution of bovine lactoferrin inter-lobe region to iron binding stability and antimicrobial activity against Staphylococcus aureus. Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua,Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua.

[17]Two novel antimicrobial peptides purified from the symbiotic bacteria Xenorhabdus budapestensis NMC-10. Xiao, Yao,Meng, Fanlu,Qiu, Dewen,Yang, Xiufen.

[18]Antimicrobial glycoalkaloids from the tubers of Stephania succifera. Zeng, Yan-Bo,Wei, Dai-Jing,Dong, Wen-Hua,Cai, Cai-Hong,Yang, De-Lan,Mei, Wen-Li,Dai, Hao-Fu,Wei, Dai-Jing,Zhong, Hui-Min.

[19]SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad spectrum antimicrobial activity. Zhu, Lei,Huang, Yan-Qing,Zhou, Jun-Fang,Fang, Wen-Hong,Yao, Xiao-Juan,Wang, Hao,Li, Xin-Cang,Zhu, Lei,Huang, Yan-Qing,Zhou, Jun-Fang,Fang, Wen-Hong,Yao, Xiao-Juan,Wang, Hao,Li, Xin-Cang,Lan, Jiang-Feng,Zhang, Chao. 2014

[20]Two New Phenolic Compounds from the Fruiting Bodies of Ganoderma tropicum. Hu, Li-Li,Ma, Qing-Yun,Huang, Sheng-Zhuo,Guo, Zhi-Kai,Guo, Jian-Chun,Dai, Hao-Fu,Zhao, You-Xing,Hu, Li-Li.

作者其他论文 更多>>

微信小程序