Design and evaluation of a polytope construct with multiple B and T epitopes against Tembusu virus infection in ducks

文献类型： 外文期刊

第一作者： Han, Kaikai

作者： Han, Kaikai;Zhao, Dongmin;Liu, Yuzhuo;Huang, Xinmei;Yang, Jing;Liu, Qingtao;An, Fengjiao;Li, Yin

作者机构：

关键词： Tembusu virus;Envelope protein;Polytope DNA vaccine;Protective immunity

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Tembusu virus (TMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in Chinese poultry industry; until now, there is no effective vaccine or drug for its prevention. Epitope-based vaccination is a promising approach to achieve protective immunity and to avoid immunopathology. In present study, based on in silico epitope selection, we optimized and proposed a polytope DNA vaccine (pVAX1-rTEM) consisting B-cell and T cell epitopes from the TMUV envelope (E) protein. The immunogenicity and protective efficacy of constructed polytope DNA vaccine was assessed by in vitro and in vivo experiments. In in vitro assays, the expressed pVAX1-rTEM showed reactivity with Tembusu positive serum. Its protective efficacy against TMUV infection was evaluated in ducks. The results showed that pVAX1-rTEM was highly immunogenic and could elicit high titer neutralizing antibodies and cell-mediated immune responses. These results indicate that pVAX1-rTEM may be a promising candidate vaccine for prevention of TMUV infection. (C) 2015 Published by Elsevier Ltd.

分类号： S85

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