Label free electrochemical aptasensor for ultrasensitive detection of ractopamine

文献类型： 外文期刊

第一作者： Wang, Peilong

作者： Wang, Peilong;Wang, Ruiguo;Su, Xiaoou;Yang, Fei;Shi, Lei;Zhou, Ying;He, Yujian;Yao, Dongsheng

作者机构：

关键词： Ractopamine;Aptamer;Graphene;Gold nanoparticles;Cyclic voltammetry;Differential pulse voltammetry

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A label free electrochemical (EC) aptasensor for ultrasensitive detection of ractopamine (RAC) was developed. A special immobilization media consisting of gold nanoparticles/poly dimethyl diallyl ammonium chloride-graphene composite (AuNPs/PDDA-GN) was utilized to improve conductivity and performance of the biosensor. The RAC aptamer was attached on AuNPs of the composite membrane via AuS bond. The fabrication process of the EC aptasensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The peak currents obtained by differential pulse voltammetry decreased linearly with the increasing of RAC concentrations and the sensor responds approximately logarithmically over a wide dynamic range of RAC concentration from 1.0 x 10(-12) mol/L to 1.0 x 10(-8) mol/L. The linear correlation coefficient of the developed aptasensor was 0.998, the limit of detection was 5.0 x 10(-13) mol/L. The proposed EC aptasensor displayed good stability, reproducibility and robust operation in animal urine. Particularly, the generality of the fabrication approach of electrochemical aptasensor is highlighted with a further example for illegal drugs detection via the aptamer identification. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q

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