Bacterioplankton community analysis in tilapia ponds by Illumina high-throughput sequencing

文献类型： 外文期刊

第一作者： Fan, Li Min

作者： Fan, Li Min;Barry, Kamira;Chen, Jia Zhang;Xu, Pao;Fan, Li Min;Hu, Geng Dong;Meng, Shun Long;Song, Chao;Wu, Wei;Chen, Jia Zhang;Xu, Pao

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关键词： Bacterioplankton community;Illumina high throughput sequencing;Tilapia ponds

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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收录情况： SCI

摘要： The changes of microbial community in aquaculture systems under the effects of stocking densities and seasonality were investigated in tilapia ponds. Total DNAs were extracted from the water samples, 16S rRNA gene was amplified and the bacterial community analyzed by Illumina high-throughput sequencing obtaining 3486 OTUs, from a total read of 715,842 sequences. Basing on the analysis of bacterial compositions, richness, diversity, bacterial 16S rRNA gene abundance, water sample comparisons and existence of specific bacterial taxa within three fish ponds in a 4 months period, the study conclusively observed that the dominant phylum in all water samples were similar, and they included; Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Planctomycetes and Chlorobi, distributed in different proportions in the different months and ponds. The seasonal changes had a more pronounced effect on the bacterioplankton community than the stocking densities; however some differences between the ponds were more likely caused by feed coefficient than by stocking densities. At the same time, most bacterial communities were affected by the nutrient input except phylum Cyanobacteria that was also affected by the feed control of tilapia.

分类号： Q93

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