Linear antigenic mapping of flagellin (FliC) from Salmonella enterica serovar Enteritidis with yeast surface expression system

文献类型： 外文期刊

第一作者： Wang, Gaoling

作者： Wang, Gaoling;Shi, Bingtian;Li, Tao;Wang, Bin;Si, Wei;Xin, Jiuqing;Liu, Siguo;Liu, Henggui;Li, Tao;Wang, Bin;Zuo, Teng;Shi, Xuanlin;Yang, Kongbin

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关键词： FIiC;Antigenic domain;Salmonella enterica serovar Enteritidis;Yeast surface expression system

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major cause of food-borne illness around the world and can have significant health implications in humans, poultry and other animals. Flagellin (FliC) is the primary component of bacterial flagella. It has been shown that the FliC of S. Enteritidis is a significant antigenic structure and can elicit strong humoral responses against S. Enteritidis infection in chickens. Here, we constructed a FIiC antigen library using a yeast surface expression system. Yeast cells expressing FliC peptide antigens were labeled with chicken sera against S. Enteritidis and sorted using FACS. The analyses of FliC peptides revealed that the FliC linear antigenicity in chickens resided on three domains which were able to elicit strong humoral responses in vivo. Animal experiments further revealed that the antibodies elicited by these antigenic domains were able to significantly inhibit the invasion of S. Enteritidis into the liver and spleen of chickens. These findings will facilitate our better understanding of the humoral responses elicited by FIiC in chickens upon infection by S. Enteritidis. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S8

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