Characterization of gonadal transcriptomes from the turbot (Scophthalmus maximus)

文献类型： 外文期刊

第一作者： Hu, Yulong

作者： Hu, Yulong;Huang, Meng;Wang, Weiji;Guan, Jiantao;Kong, Jie

作者机构：

关键词： turbot;transcriptome;sex;single nucleotide polymorphism.

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

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收录情况： SCI

摘要： The mechanisms underlying sexual reproduction and sex ratio determination remains unclear in turbot, a flatfish of great commercial value. And there is limited information in the turbot database regarding genes related to the reproductive system. Here, we conducted high-throughput transcriptome profiling of turbot gonad tissues to better understand their reproductive functions and to supply essential gene sequence information for marker-assisted selection programs in the turbot industry. In this study, two gonad libraries representing sex differences in Scophthalmus maximus yielded 453 818 high-quality reads that were assembled into 24 611 contigs and 33 713 singletons by using 454 pyrosequencing, 13 936 contigs and singletons (CS) of which were annotated using BLASTx. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses revealed that various biological functions and processes were associated with many of the annotated CS. Expression analyses showed that 510 genes were differentially expressed in males versus females; 80% of these genes were annotated. In addition, 6484 and 6036 single nucleotide polymorphisms (SNPs) were identified in male and female libraries, respectively. This transcriptome resource will serve as the foundation for cDNA or SNP microarray construction, gene expression characterization, and sex-specific linkage mapping in turbot.

分类号： Q3`R394

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