A point mutation in the glutamate-gated chloride channel of Plutella xylostella is associated with resistance to abamectin

文献类型: 外文期刊

第一作者: Wang, X.

作者: Wang, X.;Wang, R.;Yang, Y.;Wu, S.;Wu, Y.;Wang, R.;O'Reilly, A. O.

作者机构:

关键词: resistance;abamectin;glutamate-gated chloride channel;target site mutation;molecular docking

期刊名称:INSECT MOLECULAR BIOLOGY ( 影响因子:3.585; 五年影响因子:3.215 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The diamondback moth, Plutella xylostella, is a global pest of cruciferous vegetables. Abamectin resistance in a field population of P. xylostella was introgressed into the susceptible Roth strain. The resulting introgression strain Roth-Abm showed 11000-fold resistance to abamectin compared with Roth. An A309V substitution at the N-terminus of the third transmembrane helix (M3) of the glutamate-gated chloride channel of P. xylostella (PxGluCl) was identified in Roth-Abm. The frequency of the V309 allele of PxGluCl was 94.7% in Roth-Abm, whereas no such allele was detected in Roth. A subpopulation of Roth-Abm was kept without abamectin selection for 20 generations to produce a revertant strain, Roth-Abm-D. Abamectin resistance in Roth-Abm-D declined to 1150-fold compared with Roth, with the V309 allele frequency decreased to 9.6%. After treatment of the Roth-Abm-D strain with 80 mg/l abamectin the V309 allele frequency in the survivors increased to 55%. This demonstrates that the A309V mutation in PxGluCl is strongly associated with a 10-fold increase in abamectin resistance in Roth-Abm relative to Roth-Abm-D. Homology modelling and automated ligand docking results suggest that the A309V substitution allosterically modifies the abamectin-binding site, as opposed to directly eliminating a key binding contact. Other resistance mechanisms to abamectin in Roth-Abm are discussed besides the A309V mutation of PxGluCl.

分类号: R47

  • 相关文献

[1]Dynamic monitoring (B versus Q) and further resistance status of Q-type Bemisia tabaci in China. Zhang, Youjun,Zheng, Huixin,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Zhang, Youjun,Zhou, Xiaomao.

[2]Resistance of American sloughgrass (Bechmannia syzigachne) populations to ACCase-inhibiting herbicides involves three different target site mutations from China. Tang, Wei,Chen, Jie,Zhou, Fengyan,Zhang, Yong.

[3]Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins. Xuan, Ning,Guo, Xia,Xie, Hong-Yan,Liu, Guo-Xia,Picimbon, Jean-Francois,Lou, Qi-Nian,Lu, Xing-Bo. 2015

[4]Effect of abamectin on root-knot nematodes and tomato yield. Qiao, Kang,Liu, Xia,Xia, Xiaoming,Wang, Kaiyun,Wang, Hongyan,Ji, Xiaoxue.

[5]Diamondback moth resistance to insecticides in Guangdong Province. Feng, X,Chen, HY,Lu, LH. 2004

[6]Fast Separation Ultra-Performance Liquid Chromatography for Determination of Pre-Column Derivative Abamectin and Ivermectin Residues in Vegetable. Li, Qiwan,Liu, Hongcheng,Li, Qiwan,Shao, Jinliang,Zou, Yanhong,Liu, Hongcheng,Zhang, Ying,Liu, Lianliang. 2011

[7]Determination of Abamectin Residue in Paddy Rice by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. He Hong-Mei,Zhao Hua,Zhang Chun-Rong,Hu Xiu-Qing,Ping Li-Feng,Wu Min,Zhang Chang-Peng,Cai Xiao-Ming,Zhu Ya-Hong,Li Zhen. 2012

[8]Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae). Zhou, Xiao-Mao,Wu, Qing-Jun,Zhou, Xiao-Mao,Zhang, You-Jun,Bai, Lian-Yang,Huang, Xiong-Ying.

[9]Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry. Huang, Jian-Xiang,Lu, Da-Hai,Wan, Kai,Wang, Fu-Hua. 2014

[10]Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae). Tian, Huixia,Yu, Shijiang,Yue, Jiansu,Ran, Chun,Yang, Juan,Ding, Lili,Liu, Haoqiang,Li, Hongjun,Cong, Lin,Ran, Chun,Liu, Bin,Chen, Fei.

[11]Impact factors of fluorescence derivatization reaction of abamectin. Xie Xianchuan,Zhang Shaohua,Wan Dongshen,Huangpu Weiguo,Yang Ting,He Xinhua. 2006

[12]Construction and evaluation of controlled-release delivery system of Abamectin using porous silica nanoparticles as carriers. Wang, Yan,Cui, Haixin,Sun, Changjiao,Zhao, Xiang,Cui, Bo. 2014

[13]Abamectin resistance in strains of vegetable leafminer, Liriomyza sativae (Diptera: Agromyzidae) is linked to elevated glutathione S-transferase activity. Lei, Zhong-Ren,Gao, Yu-Lin,Wei, Qing-Bo,Cai, Du-Cheng,Nauen, Ralf. 2015

[14]Stage-Specific Expression of Resistance to Different Acaricides in Four Field Populations of Tetranychus urticae (Acari: Tetranychidae). Tang, Xiaofeng,Zhang, Youjun,Wu, Qingjun,Xie, Wen,Wang, Shaoli.

[15]Laboratory studies on the effect of the bioinsecticide GCSC-BtA on mortality and feeding of diamondback moth Plutella xylostella L. (Lepidoptera : Plutellidae) larvae on cabbage. Liu, B.,Zhu, Y. J.,Sengonca, C.. 2006

[16]Cross-resistance and biochemical mechanisms of abamectin resistance in the western flower thrips, Frankliniella occidentalis. Chen, Xuelin,Yuan, Linze,Du, Yuzhou,Wang, Jianjun,Zhang, Youjun. 2011

[17]Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA against Plutella xylostella L. (Lep., Plutellidae). Sengonca, C,Liu, B,Zhu, YJ. 2006

[18]Wikstroelide M potently inhibits HIV replication by targeting reverse transcriptase and integrase nuclear translocation. Zhang Xuan,Yang Liu-Meng,Chen Huan,Zheng Yong-Tang,Huang Sheng-Zhuo,Zhao You-Xing,Zhang Xuan,Zhang Xuan,Gu Wan-Gang,Wan David Chi-Cheong,Zheng Chang-Bo. 2014

[19]Specific involvement of two amino acid residues in cis-nerolidol binding to odorant-binding protein 5 AlinOBP5 in the alfalfa plant bug, Adelphocoris lineolatus (Goeze). Gu, S. -H.,Guo, Y. -Y.,Zhang, Y. -J.,Han, L.,Zhou, J. -J.. 2013

[20]Binding interactions of halogenated bisphenol A with mouse PPAR alpha: In vitro investigation and molecular dynamics simulation. Zhang, Jie,Li, Tiezhu,Wang, Tuoyi,Li, Zhuolin,Wang, Yongzhi,Wang, Yongjun,Guan, Tianzhu,Zhang, Tiehua,Yu, Hansong. 2018

作者其他论文 更多>>