Molecular and Cytogenetic Characterization of a Powdery Mildew-Resistant Wheat-Aegilops mutica Partial Amphiploid and Addition Line
文献类型: 外文期刊
第一作者: Liu, Cheng
作者: Liu, Cheng;Gong, Wen-Ping;Li, Gen-Ying;Han, Ran;Li, Hao-Sheng;Song, Jian-Min;Liu, Ai-Feng;Cao, Xin-You;Chu, Xiu-Sheng;Huang, Cheng-Yan;Zhao, Zhen-Dong;Liu, Jian-Jun;Li, Guang-Rong;Yang, Zu-Jun
作者机构:
关键词: Addition line;Aegilops mutica;Molecular marker;Partial amphiploid
期刊名称:CYTOGENETIC AND GENOME RESEARCH ( 影响因子:1.636; 五年影响因子:1.943 )
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年卷期:
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收录情况: SCI
摘要: Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA) 8 as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing. EST-based molecular marker data suggested that the wheat-Ae. mutica addition line carries the 7T chromosome. Resistance tests indicated that both the partial amphiploid and the 7T addition line were highly resistant to powdery mildew, whereas the wheat control line Chinese Spring was highly susceptible, indicating the presence of a potentially new powdery mildew resistance gene on the Ae. mutica 7T chromosome. The karyotype, FISH patterns, and molecular markers can now be used to identify Ae. mutica chromatin in a wheat background, and the 7T addition could be used as a new powdery mildew resistance source for wheat breeding. (C) 2016 S. Karger AG, Basel
分类号: Q1
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