cAMP/PKA Signaling Pathway Induces Apoptosis by Inhibited NF-kappa B in Aluminum Chloride-Treated Lymphocytes In Vitro
文献类型: 外文期刊
第一作者: Xu, Feibo
作者: Xu, Feibo;Wang, Jing;Cao, Zheng;Song, Miao;Fu, Yang;Li, Yanfei;Zhu, Yanzhu
作者机构:
关键词: AlCl3;Lymphocytic apoptosis;cAMP/PKA signal pathway;NF-kappa B
期刊名称:BIOLOGICAL TRACE ELEMENT RESEARCH ( 影响因子:3.738; 五年影响因子:3.44 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: To explore the apoptosis mechanism in lymphocytes of rats induced by aluminum chloride (AlCl3) by activating cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling pathway, the splenic lymphocytes of rats were cultured and exposed to different concentrations of AlCl3 for 24 h. The final concentrations of AlCl3 (AlCl3 center dot A 6H(2)O) in supernatant were 0 (control group, CG), 0.3 mmol/L (low-dose group, LG), 0.6 mmol/L (mid-dose group, MG), and 1.2 mmol/L (high-dose group, HG), respectively. Lymphocytes Apoptosis rate, intracellular cAMP content, PKA, survivin, B cell lymphoma/leukemia-2 (Bcl-2) and Bcl-2-associated X protein (Bax) mRNA expressions, and the mRNA and protein expressions of nuclear factor-kappa-gene binding (NF-kappa B, p65) were detected, respectively. The results showed that apoptosis index of lymphocytes, cAMP content in intracellular and PKA mRNA expression were significantly upregulated, whereas NF-kappa B and survivin mRNA expressions, nuclear NF-kappa B (p65) protein expression, and the ratio of Bcl-2 and Bax mRNA expression were downregulated in the AlCl3-treated groups compared with those in CG. The results indicated that the activated cAMP/PKA signaling pathway induces apoptosis by inhibited NF-kappa B in AlCl3-treated lymphocytes in vitro.
分类号: Q5
- 相关文献
作者其他论文 更多>>
-
4D DIA proteomic analysis of gender and age influences on meat quality and flavor in Hetian white sheep
作者:Wang, Jing;Wu, Yun;Wang, Jing;Lin, Hengxun;Zhang, Chunhui;Wang, Quanfeng;Zhang, Chunhui
关键词:Hetian white sheep; Mutton; Proteomic; Meat quality; Flavor
-
Systematic characterization of the bZIP gene family in Colletotrichum siamense and functional analysis of three family members
作者:Yang, Hong;Liu, Yu;Song, Miao;Wu, Wei;Lin, Chunhua;Yang, Hong;Huang, Weiyuan;Fan, Songle;Xue, Wenxuan;He, Qiguang;Wang, Li Feng;Huang, Weiyuan;Xue, Wenxuan
关键词:Colletotrichum siamense; bZIP transcription factors; Genome-wide identification fungicide; resistance; Virulence
-
Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi
作者:Wang, Meng;Yu, Lei;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Liu, Jiaxiao;Han, Yun;Chen, Shibao
关键词:Cadmium toxicity; Plant growth; Root exudates; Pseudomonas; Chemotaxis
-
Effect on heat treatment on the physical stability, interfacial tension and in vitro digestion of whey protein-stabilized w-6/w-3 w-3 fatty acid balanced pumpkin seed oil complex condensate
作者:Fu, Yini;Wang, Jing;Liu, Xiaoxiao;Ni, Yongqing;Niu, Zhiya;Wei, Changqing;Fu, Yini;Fu, Yini;Wang, Jing;Liu, Xiaoxiao;Ni, Yongqing;Niu, Zhiya;Wei, Changqing;Fu, Yini;Wang, Jing;Liu, Xiaoxiao;Ni, Yongqing;Niu, Zhiya;Wei, Changqing;Qiu, Ligong;Liu, Zhanxia
关键词:Heat treatment; Complexes; Oil/water interface; Interfacial properties; Pendant drop tensiometry; In-vitro simulated digestion
-
Dual Regulation of Ionic Effect on Zostera marina L. Seed Germination and Leaf Differentiation in Low-Salinity Conditions
作者:Li, Peilong;Gao, Yaping;Jiang, Zengjie;Wang, Linjie;Li, Peilong;Jiang, Zengjie;Sun, Xiaoli;Wang, Jiaqi;Wang, Jing;Sui, Haidong;Wang, Junwei;Zhang, Yitao
关键词:germination; leaf differentiation; osmotic pressure; biochemical traits; antioxidant enzymes
-
EDTA and citric acid on the growth of sunflower (Helianthus annuus L.) under chromium stress: a meta-analysis
作者:Wang, Wenjun;Huang, Xutang;Zhou, Fei;Ma, Jun;Wang, Jing;Guo, Zhenhua
关键词:Cr; Ethylene Diamine Tetraacetic Acid; network meta-analysis; phytoremediation; soil
-
Icariin-loaded chitosan/β-glycerophosphate thermosensitive hydrogel enhanced infection control and bone regeneration in canine with infectious bone defects
作者:Shao, Bing;Fu, Yang;Li, Bo;Huo, Siming;Du, Jiayu;Zhang, Xuliang;Li, Yanfei;Cao, Zheng;Song, Miao;Fu, Yang;Shao, Bing;Yin, Xin
关键词:Icariin; thermosensitive hydrogel; infection control; angiogenesis; bone regeneration