A novel C-type lectin is involved in the innate immunity of Macrobrachium nipponense

文献类型： 外文期刊

第一作者： Xiu, Yunji

作者： Xiu, Yunji;Wang, Yinghui;Bi, Jingxiu;Liu, Yuhan;Ning, Mingxiao;Liu, Hui;Li, Shuang;Gu, Wei;Wang, Wen;Meng, Qingguo;Xiu, Yunji;Wang, Yinghui;Bi, Jingxiu;Liu, Yuhan;Ning, Mingxiao;Liu, Hui;Li, Shuang;Gu, Wei;Wang, Wen;Meng, Qingguo;Xiu, Yunji;Gu, Wei;Meng, Qingguo

作者机构：

关键词： Macrobrachium nipponense;C-type lectins;Pattern-recognition receptor;Innate immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： C-type lectins (CTLs) play important roles in invertebrate innate immunity by recognizing and eliminating pathogens. In the present study, a low-density lipoprotein receptor class A (LDLa) domain-containing CTL, was identified from the oriental river prawn Macrobrachium nipponense, designated as MnCTLDcp1. The full-length cDNA of MnCTLDcp1 was composed of 1462 bp, with a 999-bp ORF encoding a 332-aa protein. An LDLa and a single C-type lectin-like domain (CTLD) were found. The mRNA transcripts of MnCTLDcp1 was expressed the highest in heart. After the prawns were challenged by Aeromonas hydrophila and Staphylococcus aureus, the expression level of MnCTLDcp1 in heart and hemocytes were all significantly up-regulated. Sugar binding assay revealed that the MnCTLDcp1 could bind to the glycoconjugates of bacteria surface, such as LPS, PGN and they can compete with bacterial as competitors. The recombinant MnCTLDcp1 agglutinates Gram-positive (S. aureus and Bacillus subtilis) and Gramnegative bacteria (A. hydrophila, Vibrio parahaemolyticus, Escherichia coli and Pseudomonas aeruginosa) in the presence of calcium and also could bind to these bacteria. These results clearly suggested that MnCTLDcp1 functions as a pattern-recognition receptor involved in the innate immunity of M. nipponense. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense. Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Liu, Xiaoqian.

[2]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[3]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[4]Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense. Jiang, S. F.,Sun, S. M.,Gong, Y. S.,Xiong, Y. W.,Qiao, H.,Zhang, W. Y.,Jin, S. B.,Fu, H. T.,Zhang, Y. P.. 2015

[5]EFFECT OF BISPHENOL A EXPOSURE ON THE EXPRESSIONS OF FIVE OVARY DEVELOPMENT RELATED GENES IN THE ORIENTAL RIVER PRAWN, MACROBRACHIUM NIPPONENSE (DECAPODA, PALAEMONIDAE). Li, Xiu,Zhang, Fengying,Jiang, Keji,Zhao, Ming,Song, Wei,Ma, Lingbo,Li, Xiu,Zhao, Weihong. 2018

[6]Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense. Jin, S. B.,Fu, H. T.,Jin, S. B.,Fu, H. T.,Jiang, S. F.,Xiong, Y. W.,Qiao, H.,Zhang, W. Y.,Gong, Y. S.,Wu, Y.. 2015

[7]Six chitinases from oriental river prawn Macrobrachium nipponense: cDNA characterization, classification and mRNA expression during post-embryonic development and moulting cycle. Zhang, Shiyong,Fu, Hongtuo,Jiang, Fengwei,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jin, Shubo,Gong, Yongsheng. 2014

[8]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[9]Calcium-calmodulin dependent protein kinase I from Macrobrachium nipponense: CDNA cloning and involvement in molting. Shen, Huaishun,Hu, Yacheng,Zhang, Yuanqin,Zhou, Xin,Xu, Zenghong,Shen, Huaishun,Hu, Yacheng,Zhang, Yuanqin.

[10]Molecular cloning of two tropomyosin family genes and expression analysis during development in oriental river prawn, Macrobrachium nipponense. Jin, Shubo,Fu, Hongtuo,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Qiao, Hui,Sun, Shengming,Zhang, Wenyi,Li, Fajun,Gong, Yongsheng,Fu, Hongtuo.

[11]Ecdysone receptor gene from the freshwater prawn Macrobrachium nipponense: Identification of different splice variants and sexually dimorphic expression, fluctuation of expression in the molt cycle and effect of eyestalk ablation. Shen, Huaishun,Zhou, Xin,Bai, Aixu,Ren, Xiufang,Zhang, Yanping,Bai, Aixu,Ren, Xiufang.

[12]Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge. Sun, Shengming,Gu, Zhimin,Gu, Zhimin,Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun.

[13]Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker. Qiao, H.,Jiang, S. F.,Sun, M.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.,Lv, D.. 2013

[14]Molecular and functional characterization of the vitellogenin receptor in oriental river prawn, Macrobrachium nipponense. Bai, Hongkun,Li, Fajun,Fu, Hongtuo,Jin, Shubo,Qiao, Hui,Fu, Hongtuo,Jiang, Sufei,Zhang, Wenyi,Xiong, Yiwei,Sun, Shengming,Jin, Shubo,Gong, Yongsheng,Wu, Yan,Yan, Yuedi,Li, Fajun.

[15]Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia. Sun, Shengming,Ge, Xianping,Fu, Hongtuo,Zhu, Jian,Xuan, Fujun,Zhang, Shiyong.

[16]Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene. Li, Fajun,Bai, Hongkun,Fu, Hongtuo,Jiang, Fengwei,Li, Fajun,Xiong, Yiwei,Jiang, Sufei,Jin, Shubo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi.

[17]Molecular cloning, characterization, and temporal expression of the clock genes period and timeless in the oriental river prawn Macrobrachium nipponense during female reproductive development. Chen, Suhua,Fu, HongTuo,Qiao, Hui,Fu, HongTuo,Sun, Shengming,Zhang, WenYi,Jin, ShuBo,Gong, Yongsheng,Jiang, Sufei,Xiong, Weiyi,YanWu.

[18]Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Zhang, W. Y.,Fu, H. T.,Zhang, Y. P.,Qiao, H.,Sun, S. M.,Jiang, S. F.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.. 2013

[19]Molecular Characterization, Genomic Organization, and Expression Analysis of Sperm Gelatinase Gene during Post-Embryonic Development in Macrobrachium nipponense. Zhang, Shiyong,Fu, Hongtuo,Bai, Hongkun,Jin, Shubo,Fu, Hongtuo,Zhang, Wenyi,Qiao, Hui,Sun, Shengming,Xiong, Yiwei,Jiang, Sufei,Gong, Yongsheng,Jin, Shubo.

[20]Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Gu, Zhimin,Xuan, Fujun.

作者其他论文 更多>>

微信小程序