The VP1 S154D mutation of type Asia1 foot-and-mouth disease virus enhances viral replication and pathogenicity

文献类型： 外文期刊

第一作者： Lian, Kaiqi

作者： Lian, Kaiqi;Yang, Fan;Zhu, Zixiang;Cao, Weijun;Jin, Ye;Liu, Huanan;Li, Dan;Zhang, Keshan;Guo, Jianhong;Liu, Xiangtao;Zheng, Haixue

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关键词： FMDV;G-H loop;Receptor recognition site;Pathogenicity;Replication

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

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收录情况： SCI

摘要： One of the proteins encoded by the foot-and-mouth disease virus (FMDV), the VP1 protein, a capsid protein, plays an important role in integrin receptor attachment and humoral immunity-mediated host responses. The integrin receptor recognition motif and an important antigenic epitope exist within the G-H loop, which is comprised of amino acids 134-160 of the VP1 protein. FMDV strain, Asia1/HN/CHA/06, isolated from a pig, was passaged four times in suckling mice and sequenced. Sequencing analyses showed that there was a mutation of the integrin receptor recognition motif Arg-Gly-Asp/Arg-Asp-Asp (RGD/RDD, VP1 143-145) and a VP1 154 serine/Asp (VP1 S154D) mutation in the G-H loop of the VP1 protein. The influence of the RGD/RDD mutation on Asia1 FMDV disease phenotype has been previously studied. In this study, to determine the influence of the VP1 S154D mutation on FMDV Asia1 replication and pathogenicity, two recombinant FMDVs with different residues only at the VP1 154 site were rescued by reverse genetics techniques and their infectious potential in host cells and pathogenicity in pigs were compared. Our data indicates that the VP1 S154D mutation increases the replication level of FMDV Asia1/HN/CHA/06 in BHK-21, IB-RS-2, and PK-15 cells and enhances pathogenicity in pigs. Through the transient transfection-infection assay to compare integrin receptor usage of two recombinant viruses, the result shows that the VP1 S154D mutation markedly increases the ability of type Asia1 FMDV to use the integrin receptors alpha(v)beta(6) and alpha(v)beta(8) from pig. This study identifies a key research target for illuminating the role of residues located at G-H loop in FMDV pathogenicity. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R1

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