Identification and characterization of immunodominant linear epitopes on the antigenic region of a serine protease in newborn Trichinella larvae

文献类型： 外文期刊

第一作者： Yang, Y.

作者： Yang, Y.;Boireau, P.;Liu, M. Y.;Yang, Y.;Vallee, I.;Lacour, S. A.;Boireau, P.;Yang, Y.;Cheng, S. P.;Liu, M. Y.

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期刊名称：JOURNAL OF HELMINTHOLOGY （ 影响因子：2.17； 五年影响因子：1.958 ）

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收录情况： SCI

摘要： An immunodominant serine protease of Trichinella spiralis named NBL1 showed encouraging potential in early diagnosis of trichinellosis in pigs and elicited protective immune responses during infection of animals. To further define serological reagents for diagnostic use, the specific epitopes on NBL protein recognized by the antibody responses of different susceptible hosts need to be defined. The present study described comprehensive mapping of immunodominant linear epitopes in the antigenic region (NBL-C, the C-terminal part of the protein) using various serum samples obtained from three kinds of hosts - pig, wild boar and mice. We identified six peptides which were commonly recognized by sera from pigs experimentally infected with Trichinella and pigs immunized with rNBL1-C; five and four peptides were recognized by sera from wild boars and mice infected with Trichinella, respectively. Three peptides (NBL1-6, -7 and -9) were commonly recognized by antisera in all three hosts, which share the sequence PSSGSRPTYP. We also found that one peptide (NBL1-12) was only recognized by antibodies from pigs immunized with rNBL1-C. The identification of specific epitopes targeted by the host antibody response is important both for understanding the natural response to infection and for the development of subunit vaccines and diagnostic tools for trichinellosis.

分类号： S852.73

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