Centimeter-sized biomimetic bone constructs fabricated via CBD-BMP2-collagen microcarriers and BMSC-gelatin microspheres

文献类型： 外文期刊

第一作者： Kou, Depeng

作者： Kou, Depeng;Kou, Depeng;Wang, Hong;Du, Mingchun;Hou, Xianglin;Chen, Bing;Li, Xing;Zhao, Yannan;Dai, Jianwu;Du, Mingchun;Fang, Yongxiang;Wang, Ling

作者机构：

期刊名称：JOURNAL OF MATERIALS CHEMISTRY B （ 影响因子：6.331； 五年影响因子：5.726 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To advance the "bottom-up" approaches for bone tissue engineering, we developed rat bone mesenchymal stem cell (BMSC)-gelatin microspheres (BGMs) as cell-culture modules and collagen binding domain-bone morphogenetic protein2 (CBD-BMP2)-collagen microcarriers (CCMs) as differentiation-control modules. BMSCs were efficiently seeded onto gelatin microspheres in a spinner flask to form BGMs with a high cell density. CBD-BMP2 was recombined and bound with collagen microfibers to form CCMs, in which BMP2 was controllably released. BGM and CCM were assembled as building blocks to fabricate macroscopic bonelike constructs in vitro and in vivo, where BMSCs were directly induced to differentiate into osteocytes. The osteogenic differentiation of BMSCs was confirmed with increasing mineral deposition (alkaline phosphatase stain, alizarin red stain, calcium content and micro-CT), gene expression of osteogenic markers (alkaline phosphatase, bone sialoprotein, osteocalcin and collagen type I) as well as alkaline phosphatase activity. The in vivo formed bone-like tissues had the compression modulus of 2.62 +/- 1.41 MPa, similar to that in the in vivo bone tissues, much higher than those in BGM samples of 0.03 +/- 0.012 MPa. Our results suggesting these proof-of-concept strategies, assembling cell-culture modules and function-control modules to modularly engineer clinical-relevant large tissue replacements, have great potential applications in tissue engineering.

分类号： O65

  • 相关文献
作者其他论文 更多>>

微信小程序